摘要
目的:探讨胶质母细胞瘤中基质金属蛋白酶(MMPs)及其组织抑制物(TIMPs)的表达,并分析其与瘤周水肿的相关性。方法:用免疫组化方法检测胶质母细胞瘤中MMP-1、MMP-2、MMP-3、MMP-7、MMP-9和TIMP-1、TIMP-2的表达,并用原位分子杂交方法检测了MMP-9mRNA分布。结果:17例胶质母细胞瘤,MMP1全部阳性,MMP-9阳性10例,主要定位于大核细胞胞核和部分胞浆;MMP-2阳性14例,MMP-3阳性6例,均位于小核细胞和细胞外基质;MMP-7阳性11例,灶性分布于出血、坏死区的小核细胞;均无TIMP-1和TIMP-2表达。8例影像学表现有瘤周水肿组MMP-9蛋白和mRNA阳性率均高于无瘤周水肿组,P<0.05,但其表达量与瘤周水肿程度之间无明确的线性关系。结论:在胶质母细胞瘤中,存在不同的MMPs的表达和TIMPs的缺乏,其中MMP-9可能与瘤周水肿的产生相关。
Objective：To investigate the expression of the Matrix Metalloproteinases （MMPs） and the Tissue Inhibitors of Metalloproteinases（TIMPs） in the glioblastoma, and analyze their correlation with the peritumor edema, nethods：Immunohistochmistry method was used to detect the expression of MMP-1, MMP-2, MMP-3, MMP-7, MMP-9 and TIMP-1, TIMP-2 in 17 cases of glioblastoma, and the in situ hybridization was used to detect the MMP-9 mRNA disribution. Results：MMP-1 positive in all the 17 cases and MMP-9 positive in 10 cases, both mainly sited in the nucleus and part of the plasma of the large nuclear cells; MMP-2 positive in 14 cases and MMP-3 positive in 6 cases, both sited in small nuclear cells and extracellular matrix; MMP-7 positive in 11 cases and locally sited in the small nuclear cells in the hemorrhagic and necrotic area; while there is no cases expresses TIMP-1 and TIMP-2. Both the ratios of MMP-9 protein and mRNA positive in the 8 cases with peritumor edema are higher than that in non-peritumor edema （P 〈 0.05）, but there is no linear correlation between the expression with the peritumor edema degrees. Conclnsions：There are varied MMPs expression and TIMPs deficiency in glioblastoma, the MMP-9 is probably related to the appearance of peritumor edema.
出处
《军医进修学院学报》
CAS
北大核心
2006年第3期214-215,共2页
Academic Journal of Pla Postgraduate Medical School
