摘要
目的提取分离赤芍中拮抗内毒素(lipopolysaccharide,LPS)的有效组分。方法采用生物传感技术结合传统的中药分离技术,分离提取中药赤芍,应用鲎试验和ELISA法检测赤芍各组分对LPS的中和作用。结果从赤芍中分离提取6个组分,其中1、4号组分对LPS具有显著的中和作用(P<0.05),在体外能够显著抑制由LPS介导小鼠RAW 264.7细胞释放TNF-α(P<0.01)。结论我们以LPS为靶点,应用生物传感器技术,快速、有效地提取分离并证明赤芍中具有较强的中和LPS活性的有效组分。
Objective To isolate and extract effective antiendotoxin materials from Radix Paeoniae Rubra. Methods By the biosensor technology, effective antiendotoxin materials were isolated and extracted with general separation technology for Chinese traditional medicine. By ELISA mensuration for LPS and inhibition of TNF-α release from endotoxin-stimulated cells in vitro, the antiendotoxin activity of Radix Paeoniae Rubra was elucidated. Results The materials extracted had high binding capability to LPS and inhibitory effect on TNF-α release from endotoxin-stimulated cells in vitro. Conclusion Isolated and extracted effective antiendotoxin materials targeting LPS by the biosensor technology were valuable in searching antiendotoxin agents from Radix Paeoniae Rubra. The ability of Radix Paeoniae Rubra to neutralize LPS was assayed and proved very high.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第13期1378-1379,共2页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目(30371767)~~
关键词
赤芍
内毒素
提取
分离
Radix Paeoniae Rubra
endotoxin
extraction
isolation
