摘要
目的:定量描述沙棘籽油对雌雄衰老小鼠脑组织中氧化还原物质的影响。方法:实验于2004-04/11在南京大学药业股份有限公司动物房完成,选择昆明小鼠128只,雌雄各半。将64只雄性小鼠按随机数字表法分为8组,分别是沙棘籽油2mL/kg,4mL/kg,6mL/kg,8mL/kg,10mL/kg,14mL/kg组、模型组和正常对照组,每组8只。籽油组分别以籽油∶糊精为1∶9,2∶8,3∶7,4∶6,5∶5,7∶3配比灌胃,正常对照组灌以蒸馏水,模型组灌以糊精,8个组灌胃剂量均为5mL/kg,每两天灌1次。除正常对照组外均用D-半乳糖进行皮下注射,1次/d,建立亚急性衰老模型。雌性小鼠64只,方法同上。灌胃45d后,分别检测雌雄小鼠脑组织中的氧化性物质丙二醛和还原性物质谷胱甘肽的含量;并建立量效函数模型。结果:128只小鼠全部进入结果分析,无脱失。①雌雄模型组小鼠脑组织的谷胱甘肽含量均显著低于正常对照组(P<0.05),6个剂量组雌雄小鼠的谷胱甘肽含量均高于模型组,沙棘籽油8mL/kg,10mL/kg,14mL/kg组与模型组相比差异具有显著性意义(P<0.05)。雌雄模型组小鼠脑组织的丙二醛含量均显著高于正常对照组(P<0.05),除雄性小鼠2mL/kg组外,其他剂量组雌雄小鼠的脑组织丙二醛含量均低于模型组,但沙棘籽油8mL/kg,10mL/kg,14mL/kg组与模型组相比差异具有显著性意义(P<0.05)。②以函数y=A2+(A1-A2)/(1+(x/X0)^p)拟合量效函数发现存在最佳剂量,雌性小鼠对药效敏感性低于雄性。结论:沙棘籽油能明显拮抗D-半乳糖的氧化损伤,在实验设置的剂量范围内较高剂量组的效果更为显著,且对雄性小鼠的药效优于雌性。
AIM： To quantitively describe the effect of seed oil of Hippophae rhamnoides L. on redox substance of brain in male and female subacute senescence-accelerated mice （SSA-mice）.
METHODS： The experiment was conducted in the animal room of the Pharmaceutical Co., Ltd of Nanjing University from April to November 2004. A total of 128 Kunming mice female and male in half were selected and the 64 male mice were randomly divided into eight groups： 2, 4, 6, 8, 10 and 14 mL/kg seed oil groups, model group and blank control group. The mice in the seed oil groups were intragastricly infused with seed oil and dextrin with a proportion of 1：9,2：8,3：7,4：6,5：5 and 7：3, respectively; the control group was infused with distilled water, and the model group with dextrin with the same dosage of 5 mL/kg, once every two days. Ex- cept the control group, the SSA-mice models were made successfully by injected D-galactose once a day. The 64 female mice were treated in the same way as the male mice. 45 days later, the content of malondialdehyde （MDA） and reduced glutathione hormone （GSH） in brain tissues were assessed to set up the does-effect function models.
RESULTS： All the 128 mice were involved in the result analysis without drop. ①The content of GSH in brain tissues of the female and male model groups were all obviously lower than the control groups （P 〈 0.05）, and the GSH in the female and male six dosages groups were all higher than the model groups, there were significant difference among the 8, 10 and 14 mL/kg groups and the model groups （P 〈 0.05）. Female and male model groups hod higher concentration of MDA compared wifh the Control groups （P 〈 0.05）, except the 2 mL/kg group, the content of MDA in the 5 dosages groups were lower than the model groups, but there were only sig- nificant difference among the 8, 10 and 14 mL/kg groups and the model groups （P 〈 0.05）.②The best dosage Was found by using the does-effect function with the equation y = A2 ＋ （A1-A2）/（1 ＋ （x/X0）^Ap. Female mice were less sensitive to drug than male mice.
CONCLUSION： The seed oil of Hippophae rhanmoides L. can obviously inhibit the senescence of mice induced by D-galactose; the higher dosage has more obvious effect in this experiment, and its effect on male mice was superior to that on female ones.
出处
《中国临床康复》
CSCD
北大核心
2006年第23期133-135,共3页
Chinese Journal of Clinical Rehabilitation
基金
南京大学创建世界高水平大学学生创新工程资助项目~~
