摘要
Preliminary studies were done on the factors affecting the callus inducement from the anther of Jartopa curcas L.,including the development stage of microspores,cold pretreatment,hormone and sucrose concentration.The results showed: compared with other stage,microspores at uninucleate stage were more suitable for culture;the cold pretreatment at 4℃ for 3～5 days is essential to high ratio of inducement;the culture medium that MS+ NAA 2.0mg/L +KT 0.4mg/L +sucrose 9% is comparativelymore suitable for callus inducement.
Preliminary studies were done on the factors affecting the callus inducement from the anther of Jartopa curcas L., including the development stage of microspores, cold pretreatment, hormone and sucrose concentration. The results showed： compared with other stage, microspores at uninucleate stage were more suitable for culture; the cold pretreatment at 4℃ for 3-5 days is essential to high ratio of inducement; the culture medium that MS ＋ NAA 2.0mg/L ＋ KT 0.4mg/L ＋ sucrose 9 % is comparativelymore suitable for callus inducement.
出处
《四川大学学报（自然科学版）》
CAS
CSCD
北大核心
2006年第3期717-719,共3页
Journal of Sichuan University(Natural Science Edition)
基金
国家"十五"重大科技攻关项目(2002BA90IA15)
