摘要
目的建立减肥保健食品中奥利司他的高效液相色谱/质谱联用检测方法。方法以含有甲酸的乙腈和水作为流动相,在反相C8柱上分离,以质谱定性,紫外定量进行检测。结果在实验条件下可以有效的扣除食品中其他成分的干扰,从而对奥利司他进行检测。结论本方法可以快速,准确地测定减肥保健食品中奥利司他的含量。
Objective To establish a method of determining orlistat in health food by on line HPLC-UV-ESl/MS. Methods The separation was completed on an analytical Spherigel C8 column（5μm, 200mm × 4.6mm） with acetonitrile （0.1% formic acid） ： water（0.1% formic acid） = 80：20 as mobile phase, the detection wavelength was 2003mm. Results Good linearity between peak areas and concentrations was obtained during rag of 0.3-0.6mg/ml. Conclusion The method is simple and can be used to determine orlistat in the health food for controlling body weight accurately.
出处
《卫生研究》
CAS
CSCD
北大核心
2006年第3期355-356,共2页
Journal of Hygiene Research
基金
"十五"国家重大科技专项"食品安全关键技术"课题资助(No.2001BA804A21
2001BA804A18-13)
