摘要
本文报道了应用甘薯茎尖分生组织培养技术获得脱毒苗的研究结果。试验表明切取的茎尖大小与脱毒效果呈显著的负相关（ｒ＝－０．９８７５），表明病毒距茎尖呈递减分布，越接近茎尖顶端带病毒越少或不带病毒。分生组织带１～２个叶原基脱毒效果较好，添加适量的病毒钝化剂可提高脱毒率，但抑制茎尖生长，成苗率有所降低。选用无症状、生长健壮的薯苗作脱毒材料，可以得到较高脱毒率的组培苗。
s The technique of producing virus free seedlings of sweet potato by the meristem tip culture was studied. The size of excised shoot tip was negative correlated with the percentage of virus free seedlings obtaind(r=-0.9875). Culture medium containing 15 mg/kg of 1.5% TS exhibited the inhibitory effect on virus multiplication. No symptom and strong sprout of sweet potato was selected as material in meristem tip culture for higher rate of virus free seedlings.
出处
《植物保护》
CAS
CSCD
北大核心
1996年第5期14-16,共3页
Plant Protection
基金
山东省计划委员会资助
关键词
甘薯
茎尖培养
脱毒
sweet potato, meristem tip culture, virus free
