摘要
目的分析人早孕期蜕膜基质细胞趋化因子配基受体对CXCL16／CXCR6的表达及免疫活性细胞趋化因子受体CXCR6的表达,以探讨CXCL16／CXCR6在蜕膜免疫活性细胞募集中的可能规律。方法收集早孕期蜕膜组织,分离蜕膜基质细胞和免疫细胞,分别采用半定量RT-PCR、免疫细胞化学、流式细胞术分析蜕膜基质细胞CXCL16和CXCR6的表达;流式细胞术分析蜕膜CD56^+CD16^- NK细胞、CD56^+CD16^+NK细胞、NKT细胞、T细胞、γδT细胞、单核细胞CXCR6的表达。结果人早孕蜕膜基质细胞高水平转录趋化因子受体CXCR6,低水平转录其配体CXCL16,但CXCL16和CXCR6在蛋白水平的表达偏低。早孕蜕膜γδT细胞CXCR6阳性率为87．29％;CD14^+单核细胞CXCR6阳性率为47．71％;NKT细胞CXCR6阳性率为44．14％;T细胞CXCR6表达率为32．91％;而蜕膜两种NK细胞 (CD56^+CD16^-、CD56^+CD16^+)几乎不表达CXCR6,,结论人γδT细胞、单核细胞、NKT细胞、T细胞可能通过表达趋化因子受体CXCR6被募集到蜕膜局部并驻留,从而参与早孕期母胎界面的免疫调节。
Objective To investigate the expression of chemokine receptor CXCR6 in decidual stromal （DSC） and decidual immunological competent cells of human early pregnancy. To evaluate the possible function of CXCL16/CXCR6 in recruitment mechanism of decidual leukceytes. Methods After decidual stromal and decidual leukocytes were isolated and purified, the trarscription and trarslation of CXCR6/CXCL16 were assessed by semi-quantitative RT-PCR, immunocytochemistry and flow cytometric assay in DSC, and the expressions of CXCR6 were examined in decidual CD56^＋CD16^- NK cell, CD56^＋ CD16^＋ NK cell, Tcell, γδT cell, NKT cell and monocyte by flow cytometry. Results Although CXCR6 was highly transcribed in purified DSC, the protein level of either CXCL16 or CXCR6 was lower in decidual stromal cell. Decidual lymphoeytes expressed chemokine receptor CXCR6 in different levels. The results showed that 87.29% of γδT cells, 48.37% of monocytes, 44.14% of NKT cells and 32.91% of T cells expressed CXCR6, but both CD56^＋ CD16^- NK cells and CD56^＋CD16^＋ NK cells expressed CXCR6 in a very low level. Conclusion CXCL16 produced by matemo-fetal interface contributes to the recruitment and residence of decidual γδT cells, T cells, NKT cells and monocytes in first-trimester deciduas.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2006年第4期298-302,共5页
Chinese Journal of Microbiology and Immunology
基金
复旦大学"985工程"项目资助
