摘要
背景与目的:研究饮水氯化消毒副产物3-氯-4-二氯甲基-5-羟基-2(5氢)-呋喃酮(3-chloro-4-(dichloromethyl)-5-hydroxy-2[5H]-furanone,MX)对体外培养的人胚胎肝细胞(L-02细胞)ras基因突变的诱导。材料与方法:MX染毒剂量为300μmol/L,以二甲基亚砜(DMSO)做溶剂对照,将L-02细胞连续染毒培养12d后,收获细胞提取基因组DNA,应用PCR-克隆测序法检测ras基因(K-ras、H-ras、N-ras)12、13、61密码子是否存在突变。结果:MX染毒组H-ras基因57密码子的GAT置换成GGT,未检测到K-ras、N-ras及H-ras12、13、61密码子突变,DMSO溶剂对照组相应的ras基因目的片段均未检测到突变。结论:MX可能诱导L-02细胞ras基因突变。
BACKGROUND ＆ Albl： To study the mutation of ras genes induced by 3-chloro-4-（diehloromethyl）-5-hydroxy-2[5H] -furanone （MX） in human fetal hepatocytes （L-02） in vitro. MATERIAL AND METHODS： L-02 cells were treated consecutively with 300 μmol/ L of MX for 12 days and genome DNA was extracted after cell harvesting.Dimethyl sulfoxide （DMSO） was used as solvent control. Ras genes（K-ras, H-ras, N-ras） sequences including 12, 13 and 61 codons were tested using the PCR-clone and sequencing with the genome DNA from the cells. RESULTS： H-ras gene 57 codon substitution from GAT to GGT in the MX-treated group was found, but 12, 13, 61 codons of K-ras,H-ras, N-ras were the same as the normal sequence. There was no mutation in the solvent control group. CONCLUSION：MX probably induced ras gene mutation of L-02 cells.
出处
《癌变．畸变．突变》
CAS
CSCD
2006年第3期171-174,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金(No.30170794)
