摘要
目的 观察慢性乙型肝炎肝纤维化及肝硬化患者肝组织病理严重程度与血清乙型肝炎病毒脱氧核糖核酸（HBV-DNA）、乙型肝炎病毒e抗原（HBeAg）的关系.方法 选择住院的慢性乙型肝炎、肝硬化患者55例,分别根据炎症及纤维化程度分为3组,行肝组织病理学检查,应用免疫荧光聚合酶链反应方法检测血清HBV-DNA的含量;应用酶联免疫吸附测定法检测血清HBeAg.结果 随炎症程度加重（G1～2,G3,G4）,HBV-DNA（对数值）逐渐下降（6.19±1.36,5.42±1.52,4.42±1.33）（P〈0.05）,HBV-DNA与炎症程度呈负相关（γs=-0.481,P〈0.01）;随纤维化程度加重（S1-2,S3,S4）,HBV-DNA亦逐渐下降（6.37±1.22,5.51±1.52,4.60±1.42）,P〈0.05,与纤维化程度呈负相关（γs=-0.501,P〈0.01）;同时HBeAg阳性率随炎症程度加重而下降（84.2%,57.1%,46.7%）（P〈0.05）,亦随纤维化程度加重而下降（87.5%,61.1%,47.6%）（P〈0.05）.结论 随肝组织病理程度加重,慢性肝炎患者血清HBV-DNA载量、HBeAg阳性率逐渐下降.
Objective To study the relationship among pathology in liver tissues and level of hepatitis B virus DNA（HBV-DNA）,hepatitis B e antigens（HBeAg） in serum of patients with chronic hepatitis B. Methods The liver biopsy was performed in 55 hospitalized patients. According to hepatitis grades（G） and fibrosis stages（S）, the patients were divided into three groups. Hepatic pathology was examined, and the serum HBV-DNA level was tested by amolisensor quantitative polymerase chain reaction and serum HBeAg by enzyme linked immunosorbent assay. Results With the development of liver inflammation from G1-2 group to G3, G4 group,the serum HBV-DNA decreased （6.19 ± 1.36,5.42±1.52,4.42 ± 1.33） （ P 〈 0. 05 ）, and HBV-DNA decreased from S1-2 group to S3, S4 group, too （6.37 ± 1.22,5.51± 1.52,4.60 ± 1.42）（ P 〈0.05）. The serum HBV-DNA was negative correlated with the hepatitis grades and fibrosis stages（ γ = - 0. 481,0. 501, respectively, P 〈 0.01）. The positive rate of serum HBeAg decreased from G1-2 group to G3, G4 group （84.2 %, 57.1 %, 46.7 % ） （ P 〈 0.05） ; and decreased from S1-2 group to S3 , S4 group, too （87.5 %,61.1% ,47.6 %）（ P 〈 0.05）. Conclusion With the development of liver fibrosis stages and grades in the patients with chronic hepatitis B,the serum level of HBV-DNA and the positive rate of HBeAg decreased.
出处
《临床荟萃》
CAS
北大核心
2006年第10期706-708,共3页
Clinical Focus
关键词
肝炎
乙型
肝硬化
肝炎e抗原
乙型
DNA复制
hepatitis B
liver cirrhosis
hepatitis B e antigens
DNA replication
