摘要
A new selfincompatibility gene was isolated and identified from Pyrus bretschneideri cultivars of Yingzhiqing and Daaoao via PCR amplification, DNA sequence analysis and cross pollination tests. DNA sequence analysis revealed that the isolated fragment displayed a high homology with S 1 ～S 11 allele, and the identity to S 1 ～S 11 allele at the deduced amino level ranged from 56% to 72%; the high degree of variances in the hypervariable （HV） region resulted from the presence of substitution, deletion and insertion of 9 to 15 amino acids. The new Sallele was named S 12 RNase and its accession number was AY250987 in GeneBank. The sizes of HV region, intron, signal peptide, C1 region, C2 region were 39 AA, 341 bp, 27 AA, 11 AA and 10 AA, respectively. The cross pollination tests were carried out using Pyrus pyrifolia cultivars that contained S 1 ～S9RNase genes as female parents, and the cultivars of Daaoao and Yingzhiqing as male parents, respectively. The results showed that all of {%P.pyrifolia%} cultivars were compatible with Daaoao and Yingzhiqing, whereas the cross pollination between Daaoao and Yingzhiqing were incompatible, further confirming that the DNA fragment isolated was a new Sgene.
A new self-incompatibility gene was isolated and identified from Pyrus bretschneideri cultivars of Yingzhiqing and Daaoao via PCR amplification, DNA sequence analysis and cross pollination tests. DNA sequence analysis revealed that the isolated fragment displayed a high homology with S1 - S11-allele, and the identity to S1 - S11-allele at the deduced amino level rangedfrom 56% to 72%; the high degree of variances in the hypervariable （HV） region resulted from the presence of substitution, deletion and insertion of 9 to 15 amino acids. The new S-allele was named S12-RNase and its accession number was AY250987 in GeneBank. The sizes of HV region, intron, signal peptide, C1 region, C2 region were 39 AA, 341 bp, 27 AA, 11 AA and 10 AA, respectively. The cross pollination tests were carried out using Pyrus pyrifolia cuhivars that contained S1- S9-RNase genes as female parents, and the cultivars of Daaoao and Yingzhiqing as male parents, respectively. The results showed that all of P. pyrifolia cultivars were compatible with Daaoao and Yingzhiqing, whereas the cross pollination between Daaoao and Yingzhiqing were incompatible, further confirming that the DNA fragment isolated was a new S-gene.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2006年第4期117-121,共5页
Scientia Silvae Sinicae
基金
国际合作项目"中国梨自交不亲和基因的分离克隆与测序"
湖南省三项基金"湖南省主要沙梨品种的自交不亲和性的研究"(00JZY2155)
国家林业局重点项目"梨自交不亲和技术的开发与应用"。
