摘要
目的探讨重组凋亡蛋白TFAR19对鼻咽癌细胞株CNE-1的促凋亡效应。方法将不同浓度的高纯度重组凋亡蛋白TFAR19分别和人类鼻咽癌细胞株CNE-1共同培养,通过细胞形态学观察,TUNEL检测及DNA片断化分析,观察TFAR19对细胞的促凋亡效应。结果不同浓度TFAR19蛋白均可促进细胞凋亡,加入5、10、15、20、30 mg/L的TFAR19蛋白后,鼻咽癌细胞株CNE-1的凋亡率分别是:15.26%、29.48%、37.11%、54.20%、72.36%。阴性对照组的细胞凋亡率为12.40%。结论TFAR19蛋白可直接进入CNE-1细胞并明显促进细胞凋亡,且呈剂量依赖性。
Objective Human recombinant TFAR19 protein apoptosis inducing effect on human nasopharyngeal carcinome ONE- 1 cells was studied. Methods Human recombinant TFAR19 protein was cultured with ONE - 1 cells and the apoptotic cells were analyzed by DNA fragmentation, TUNEL and cell morphology. Results Different concentrations of TFAR19 significantly promoted apoptosis of ONE 1 cells, and the dose - dependent percentages of cell apoptosis were ranged from 15.26% to 72.36%. Conclusion Human recombinant TFAR19 protein can enter ONE 1 cells and induce apoptosis of CNE - 1 cells.
出处
《实用预防医学》
CAS
2006年第2期222-223,共2页
Practical Preventive Medicine
基金
湖南省卫生厅科研基金(B2005-066)
