摘要
筛选了一株产纤溶酶能力较强的芽孢细菌,本文对其液体发酵条件进行了优化,结果表明菌株产酶最佳条件为:可溶性淀粉4%,蛋白胨2%,柠檬酸铁铵0.1%,磷酸钙0.4%,接种量2%,pH 7.5,发酵时间96 h,装瓶量75/500(mL/mL),摇床温度37℃,转速150 rpm,在该条件下产酶酶活可达581.81 IU/mL。
A fibrinolytie enzyme-produing bacillus was screened in our experiment. The optimization of fermentation conditions for the high-yield strain indicated that the optimal conditions were： soluble starch 4%, peptone 2%, ammonium ferric citrate 0.1%, calcium phosphate 0.4%, the inoeulum size 2%, pH 7.5, fermentation time 96 h, media amount 75/500 （mL/mL）, fermentation temperature 37 ℃, rotate speed 150 rpm. Under these eonditions, the strain＇ s flbrinolytie enzyme activity was 581.81 IU/mL.
出处
《天然产物研究与开发》
CAS
CSCD
2006年第2期203-206,共4页
Natural Product Research and Development
关键词
纤溶酶
酶活力
发酵条件优化
正交实验
fibfinolytic enzyme
enzyme activity
optimization of fermentation conditions
direct cross test
