摘要
目的研究小儿在轮状病毒急性感染期的免疫应答特点。方法采用ELISA方法检测血浆和粪便轮状病毒特异性抗体,流式细胞术进行淋巴细胞亚群分析,采用荧光定量RTPCR方法检测外周血单个核细胞转录因子、细胞因子mRNA的表达。结果腹泻患儿血浆中轮状病毒特异性IgMIgG抗体和大便中轮状病毒特异性IgA抗体及CD19细胞亚群比例,均较对照组有明显的升高。腹泻患儿外周血单个核细胞中IL12p40mRNA的表达升高。粪便和血浆中IgA抗体的滴度与患儿腹泻的严重程度有一定关系。结论急性轮状病毒感染小儿早期出现的免疫应答反应,以特异性抗体显著增加为主要特点,并且抗体产生的水平与疾病的严重程度相关。
Objective To assess immune response in children with acute rotavirus dianhea. One hundred and two children with acute rotavirus dianhea and 45 control children were enrolled in this study during Sep. 2004 to Jan. 2005. ELISA assay was developed to determine rotavirus-specific antibody liter in plasmas and stools. Subgroups of lymphocytes were examined by flow cytometry. The levels of mRNA expression of transcription factors （T-bet, GATA-3） and cytokines（IFN-У, IL-2, IL-4, IL-12p40） were examined by fluorescent quantitative RT-PCR. Eighty-two patients had detailed documents of clinical characteristics. Results Compared with control, the percentage of CD19^＋ cells in PBMC of the patients was increased by 7.7%, P = 0.009. The children with diarrhea had higher median d rotavirus specific IgM/IgG titer in plasmas and IgA titers in stools than control, P 〈0.05. Patients with acute rotavirus infection had elevated mean expression levels of IL-12p40 mRNA in PBMC, but no obvious changes were found in other transcription factors and cytokines. Rotavirus specific IgA fiters in stools were higher in patients with more severe diarrhea inchding those with longer durations or increased fecal frequencies. Patients with vomiting had significantly higher liters of plasma IgA than those without vomiting. Patients younger than 12 months had more duration days of diarrhea and also elevated IgA titers in stools. Conclusion Production d rotavims-specific antibody is critically important in host immune response against acute rotavirus infection, and the levels d antibody is associated with the severity d disease.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2006年第3期248-251,共4页
Chinese Journal of Microbiology and Immunology
