摘要
[目的]通过观察重组人骨形态发生蛋白-2(rhBMP-2)诱导成骨过程中神经营养因子(NTFs)家族及受体的表达,探讨神经营养因子在BMP骨诱导中的作用。[方法]建立小鼠右侧股后部异位成骨模型,实验组植入rhBMP-2胶原复合物,对照组仅植入相同体积的胶原海绵。分别于术后7、14和21 d取材,进行组织学、免疫组化及RT-PCR检测。[结果]组织学证实rhBMP-2胶原复合物具有良好的骨诱导能力,而对照组未见成骨。术后第7 d,在大量软骨形成期NGF和TrkA阳性染色达到高峰,成纤维细胞、成软骨细胞、软骨细胞、肥大软骨细胞和成骨细胞中均有阳性表达;BDNF在软骨细胞及软骨基质中有阳性染色,其受体TrkB仅在成软骨细胞和软骨细胞中有表达;NT-3与NGF表达相似,其受体TrkC仅在成软骨细胞和软骨细胞中有阳性染色。术后第14 d,NGF和TrkA阳性表达局限于部分成骨细胞、骨样细胞和成骨样细胞;NT-3在软骨细胞、成骨细胞中明显表达。术后21 d,只有少量成骨细胞、成骨样细胞中有NGF和TrkA的表达。RT-PCR检测结果显示,NTFs mRNA于术后第7 d表达最高,与免疫组化中蛋白质的表达结果相一致。[结论]NTFs及其受体在rhBMP-2诱导成骨过程明显表达,提示NTFs可能通过直接和间接的方式协同BMP的诱导成骨过程。
[ Objective] To identify the expression of neurotrophic factors （NTFs） and their high affinity receptors （Trk） during the bone induction by recombined human hone morphogenetic protein-2 （rhBMP-2） and discuss possible roles of NTFs on the osteoinductive activity of BMP. [ Method] After a total of 36 ICR mice were divided into experimental group and control group at random, rhBMP-2/collagen sponge and collagen sponge were implanted into the right thigh muscle pouches of two groups respectively. Tissues in the implanted sites of two groups were removed on the 7^th, 14^th and 21^st day after implantation. Histological, immunohistochemical and RT-PCR analyses were performed to detect osteoinductive effect of rhBMP- 2 and the expression of NTFs and Trk. [ Result] RhBMP-2/collagen sponge displayed a potent ability inducing bone formation, while the expression of NTFs and Trk was observed in the course of osteoinduction by rhBMP-2. Especially on the 7^th day in the experimental group, NGF and TrkA positive immunostaining reached the peak in the stage of chondrogenesis, including chondroblasts, chondrocytes, hypertrophic chondrocytes and osteoblasts, then decreased in the number of positive cells and the intensity of immunostaining on the 14^th and 21^st day. BDNF was only observed in chondrocytes and cartilage matrix, and TrkB was expressed in chondreblasts and chondrocytes at the 7^th day after implantation. Expression of NT-3 was similar to that of NGF, while TrkC was expressed in chondroblasts and chondrocytes on day 7. Expression level of the mRNA of NTFs peaked at 7 days after the implantation, then decreased, which was nearly coincident with immunohistochemical results. [ Conclusion ] Many different kinds of cells are induced by rhBMP-2 express NTFs and Trk, which suggests that NTFs may play an important role inthe osteogenesis initiated by BMP through direct and indirect pathways.
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2006年第7期535-537,i0001,共4页
Orthopedic Journal of China
关键词
骨形态发生蛋白
骨诱导
神经营养因子
受体
Bone morphogenetie protein
Osteoinduction
Neurotrophic factors
Receptor
