摘要
用毛细管气相色谱法测定烟草中烟碱、降烟碱、麦斯明、二烯烟碱、新烟碱、去氨新烟碱、2，3＇-联二吡啶、可替宁8种主要生物碱的方法。烟草样品经二氯甲彬甲醇（V／V，3：1）萃取，过一次性滤膜，进样，经DB-5MS毛细管柱分离，由气相色谱-氢火焰离子化检测器（FID）检测定量，质谱定性。该方法操作简单，重现性好，回收率较高。8种生物碱相对标准偏差为2．59％-7．07％；回收率为89．4％-98．7％。
A gas chromatographic method for analysis of 8 nicotine-related alkaloids in tobacco including nicotine, nomicotine, myosmine, nicotyrine, anabasine, antabine, 2,3＇-dipyridyl and cotinine was devel- oped.0. 3 gram flue-cured tobacco and 1.5 mL 6% ammonia were placed in a glass bottle with a lid, and the bottle was put in an ultrasonic bath for 2min. Methylene chloride/methanol of 5 mL （ V/V,3：1 ） was added into the bottle and the two layers were separated. 2μL filtrate of chloride/methanol layer was quantitatively analyzed by capillary column （DB-5MS） gas chromatography equipped with flame ionization detector （FID） and qualitatively analyzed by mass spectrometry. This simple procedure provided good precision （ RSDs = 2. 6% -7.1% ） and good recoveries of 89.4% -103.4%.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2006年第3期382-384,共3页
Chinese Journal of Analytical Chemistry
基金
国家烟草局科学基金资助项目(No.110200401047
110200302028)
关键词
烟草分析
生物碱
萃取
气相色谱/质谱
Tobacco, alkaliods, extraction, gas chromatography-mass spectrometry
