摘要
目的:建立以高效液相色谱法测定复方维生素胶囊中维生素B12含量的方法。方法:色谱柱为HypersilODS,流动相为甲醇-0.05mol/L磷酸二氢钾缓冲液(22∶78,pH=6.4),流速为1.0ml/min,检测波长为360nm,柱温为室温,进样量为20μl。结果:维生素B12进样量在10.20μg～204.00μg范围内与峰面积积分值线性关系良好(r=0.9999),平均回收率为99.7%(RSD=1.18%)。结论:本方法简便、快速、准确,不受维生素B6、叶酸等成分的干扰,可用于测定复方维生素胶囊中维生素B12的含量。
OBJECTIVE： To establish a HPLC method for determination of Vitamin B12 in compound vitamin capsules. METHODS： Compound vitamin capsules were determined by HPLC on a ODS column at room temperature.The mobile phase consisted of methyl alcohol and 0.05mol/L KH2PO4 buffer（22 ： 78,pH 6.4）. The flow rate was 1.0ml/min,and the detection wave length was set at 360nm.The sample size was 20μl.RESULTS： The sample size of vitamin B12 presented a good linear relationship with peak area in the range of 10.20μg～204.00μg（r=0.9 999） .The average recovery was 99.7% （RSD = 1.18% ）.CONCLUSIONS：The present method is simple,convenient,and accurate for determination of vitamin B12 in compound vitamin capsules,and it also has the advantage of preventing the interference from vitamin B6 and folic acid.
出处
《中国药房》
CAS
CSCD
北大核心
2006年第6期460-461,共2页
China Pharmacy
