摘要
家蚕自3龄起添食单一和混合的Cd2+、Pb2+重金属溶液,观察并记录其生长发育状况,测量各时期体长,提取DNA并分析断裂情况。结果表明,添食Pb2+、Cd2+两种混合重金属离子的家蚕体型比单一添食相应含量Cd2+的大,其体长大于单一添食相同含量Pb2+、Cd2+的家蚕;单一重金属离子作用下的家蚕DNA发生明显的梯状断裂,出现细胞凋亡现象,而混合重金属诱导的家蚕细胞DNA完整,未发生凋亡现象,预示着竞争位点假说在家蚕体内的存在。
The growth and development state and DNA disruption of silkworm, fed on the mulberry leaves treated with Pb^2＋ , Cd^2＋ or mixture of them since the 3^rd instar, were investigated. The results showed that the body figure and length of silkworm fed on the mixture were larer and longer than those of silkworm fed on Pb^2＋ or Cd^2＋. The electrophoresis results of DNA from haemolymph indicated that specific DNA bands appeared clearly and regularly for silkworms fed on Pb^2＋ or Cd^2＋, but for silkworms fed on the mixture. This suggested that there existed the apoptosis only in haemolymph of silkworms fed on Pb^2＋ or Cd^2＋, The above results suggested that the hypothesis of rivalship among heavy metal ions existed in the silkworm.
出处
《蚕业科学》
CAS
CSCD
北大核心
2006年第1期125-128,共4页
ACTA SERICOLOGICA SINICA
基金
苏州市自然科学基金项目(编号SNZ-0221)
