摘要
研究了辣根过氧化物酶（HRP）／820，体系中苯酚与4-氨基安替比林（4-AAP）的偶合反应。在pH8.0的条件下，产物在494．0nm处有最大吸收，线性范围为（4．3×10^-6-2．6×10^-4）mol／L（R^2=0．9993），检出限为4．75×10^-7mol／L。相对标准偏差为3．7％（n=9）。动力学方面，用孤立法研究了各反应物对反应速率的影响，得到4-AAP、H2O2和phenol的反应级数分别为0、1．3和0．7，据此建立了反应速率方程式，求得活化能为42．09kJ/mol，并推测出反应的可能机理。
A colorimetric system involving horseradish peroxidase （HRP）, hydrogen peroxide and 4-aminoantipyrine （4-AAP） for the determination of phenol was developed and optimized. The method was based on the coupled reaction between 4-AAP and phenol in alkaline medium initiated by a horseradish peroxidase-catalyzing redox system and the absorbance of the red product at 494.0 nm was proportional to the amount of phenols. The linear detection range was （4.3 × 10^-6 - 2.6 × 10^-4） mol/L （ R^2 = 0.9993）. The detection limit was 4.75 × 10^-7 mol/L with 3.7% RSD. for n = 9 （at 4.3 × 10^-6 mol/L）. The orders of all reacting substances were obtained at 20 ℃ by spectrophotometer. The activation energy was calculated as 42.09 kJ/mol. Based on experimental and theoretical data, a mechanism was given. This method was applied to the analysis of water samples.
出处
《分析试验室》
CAS
CSCD
北大核心
2006年第3期46-49,共4页
Chinese Journal of Analysis Laboratory
基金
陕西省自然科学基金(2003B13)
