摘要
氯霉素滴眼液中常含有抑菌剂尼泊金乙酯，对直接紫外分光光度法测定含量往往产生干扰。使用普通型（手动式）紫外分光光度计，结合作者开发研制的计算机导数紫外测定程序，经微机数据处理及图形分析，采用一阶导数分光光度法，不需提取分离，直接取样测定，就可消除尼泊金乙酯的干扰，便于快速分析。测定波长为２９８ｎｍ和３０２ｎｍ，在１５５×１０－２～７７５×１０－２ｍｍｏｌ／Ｌ的浓度范围内线性关系良好（ｒ＝０９９９９５），平均回收率和变异系数分别为１００４８％和０５４％
The measuring accuracy of chloramphenicol content in its eye drops is influenced by existence of the ethyl p hydroxyhenzoate in traditional ultraviolet spectrophotometric method.A common ultraviolet spectrophotometer combined with computer program is given.By use of first order derivative spectrophotometry and data processing and graph analysis,an appropriate result not interfered with ethyl p hydroxyhenzoate is obtained at the 302 and 298 nm wavelengths from the direct sample.A good linearity has shown in measuring within the range of 1.55×10 -2 ～7.75×10 -2 mmol/L( r =0 999 95).The average recovery and the coefficients of variation are 100.48%and 0 54%respectively.
出处
《北京联合大学学报》
CAS
1996年第1期45-48,共4页
Journal of Beijing Union University
关键词
氯霉素滴眼液
氯霉素
尼泊金乙酯
分光光度法
chloramphenicol eye drops
chloramphenicol
ethyl p hydroxyhenzoate
first order derivative spectrophtometry
determination
