摘要
目的:探讨生存素(Survivin)、p27蛋白在人宫颈癌中的表达水平与宫颈癌组织侵袭、转移能力的关系。方法:采用免疫组织化学S-P法和图像分析系统检测Survivin、p27在10例正常宫颈、10例宫颈原位癌、40例宫颈鳞癌、11例宫颈腺癌组织中的表达,分析表达结果与临床病理特征的关系。结果:从正常宫颈上皮→原位癌→浸润癌,Survivin阳性表达量显著升高,p27表达量显著降低(P<0．05)。宫颈癌Survivin表达量与盆腔淋巴结转移、局部侵袭、组织学类型、患者年龄有关(P<0．05),而与FIGO分期、组织学分级无关(P>0．05);宫颈癌p27阳性表达量与盆腔淋巴结转移、局部侵袭、组织学分级有关(P<0．05),而与FIGO分期、组织学类型、患者年龄无关(P>0．05)。有盆腔淋巴结转移、脉管和(或)间质侵袭患者的Survivin阳性表达量增多,而p27的表达量与之相反(P<0．05)。结论:Survivin、p27蛋白异常表达在宫颈癌中的恶化、侵袭、转移中起重要作用,检测Survivin、p27蛋白表达水平,对判断宫颈癌的恶性程度、侵袭转移潜能,评估患者预后及制定优化治疗方案有一定参考价值。
Objective： To investigate the relationship between survivin and p27 in the process for gene protein expression to invasion and node metastasis in cervical carcinoma. Methods： The expression of survivin and p27 was examined by irnmunohistochemical SP method and colour pathological image computer analysis system in 10 cases of normal cervical epithelia, 10 cases of cervical carcinoma in situ, 40 cases of invasive squamous cell carcinoma of cervix and 11 cases of invasive andeocarcinoma of cervix. The relationship between the two indexes and the factors related with clinical pathology of cervical carcinoma was analyzed statistically. Results： It was found that the positive level of survivin expression followed this order： normal cervical epithelium→ cervical carcinoma in situ→ invasive carcinoma of cervix; while the expression of p27 were in reserve order （P〈0. 05）. There was significant correlation between the expression of survivin to pelvic lymphonode metastasis, intravascular involvement, stroma involvement and histopathological types and ages of patients （P〈0. 05）, but no correlation between them to FIGO staging and histropathological grading （P〈0. 05）. There was significant correlation between the expression of p27 to pelvic lymphonode metastasis, intravascular involvement, stroma involvement and histropathological grading （P〈0. 05）, but no correlation between them to FIGO staging, histopathological types and ages of patients （P〉0. 05）. The positive expression of survivin in patients with pelvic lymph node metastasis, intravascular involvement, or stroma involvement were significantly higher than those in the cases without them, but the positive expression of p27 had the contrary result with survivin positive expression. Condusion：The positive expression of survivin and p27 in invasive carcinoma of cervix might play an important role during the development, lymph node metastasis, intravascular and stroma involvement of cancer cells, and they had affinity with clinical behavior of cervical carcinoma. Detecting the expressive levels of suvivin and p27 have certain referent values for judging the malignant degree, potentials of invasion and node metastasis in cervical carcinoma, evaluating prognosis of patients and selecting the rational theraputic protocol after surgery.
出处
《中国临床医学》
北大核心
2006年第1期105-107,共3页
Chinese Journal of Clinical Medicine
基金
湖北省教育厅科研基金(2004D010)十堰市科技攻关课题(2004zd007)
关键词
宫颈癌
生存素
P27
免疫组化
肿瘤转移
Cervical carcinoma, Survivin
p27
Immunohistochemistry
Tumor metastasis
