摘要
目的建立适合于临床发病机制研究和药物筛选的动物子宫肌瘤模型。方法取性成熟豚鼠,双侧摘除卵巢,以不同频率给予不同剂量雌激素(E2)诱导,使之形成子宫肌瘤,并于不同时间卒死动物,观察子宫形态的变化,计算成瘤率,并与人子宫肌瘤病理组织学特征相比较,确定E2诱导子宫肌瘤形成的最佳剂量、给药频率和实验周期。同时测定血清中雌(E2)、孕激素(P)浓度及组织中雌、孕激素受体(ER、PR)的表达。结果皮下注射E2100μg/只,2次/wk,连续12 wk后,豚鼠子宫明显变形,肌层增生,肉眼可见浆膜下肌瘤或肌壁间瘤形成,并伴有继发性改变。病理组织学检查结果显示,肌瘤细胞呈长梭形,编织状或栅状排列,细胞核呈短棒状或卵圆形,显示明显的子宫肌瘤特征,与人子宫肌瘤病理组织学特征相似。血清E2浓度升高,组织中雌、孕激素受体表达明显增强。结论双侧摘除卵巢的豚鼠皮下注射E2100μg/只,2次/wk,连续12 wk后可建立与人类相似的动物子宫肌瘤模型,适合用于临床发病机制研究和药物筛选。
Aim To establish an animal model of uterine leiomyoma for pathogenesis research and drug screen analysis. Methods Guinea pigs were treated with estradiol benzoate 100 μg ·d^ -1 , twice a week, by intramuscular injection for 12 weeks. After the animal were killed, the uteri were dissected out and ultrathin sections were obtained. Compare to that of human being, pathohistologic characteristics were observed under the microscope. Results The uteri treated with estrogen were proliferated and deformated. Uterine nodules were observed in many of the tested animals. According to the results of pathohistology, these cells demonstrate the features of smooth muscle-like cells. Conclusion The uterine leiomyoma induced by estrodiol in guinea pig is similar to that of human being. It has been suggested that the animal model is valuable for pathogenesis research and drug screen analysis, although it still needs more evidence to be demonstrated.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2006年第3期374-378,共5页
Chinese Pharmacological Bulletin
关键词
子宫肌瘤
动物模型
豚鼠
雌激素
uterine leiomyoma
animal model
guinea pig
estrogen
