摘要
目的观察外周血中性粒细胞黏附分子CD11b/CD18的表达水平与下肢动脉硬化闭塞症(ateriosclerosisobliterans,ASO)的关系及体外血府逐瘀口服液对CD11b/CD18的影响。方法应用流式细胞术直接免疫荧光法,检测ASO患者及健康对照组外周血中性粒细胞黏附分子CD11b/CD18的表达水平;分离培养患者中性粒细胞,用血府逐瘀口服液干预,检测用药后(1h、6h、12h)CD11b/CD18的表达水平的变化。结果ASO患者CD11b/CD18的表达水平显著高于对照组(P<0.05),与病情严重程度一致,随着病情的进展CD11b/CD18的表达增高;体外用血府逐瘀口服液干预6、12h后中性粒细胞CD11b/CD18的表达降低,与未用药组及用药1h组比较,差异有显著性(P<0.05)。结论CD11b/CD18可能参与了ASO的发病过程,并且与动脉硬化程度有关,血府逐瘀口服液治疗和预防ASO的机制之一,可能是通过降低外周血中性粒细胞CD11b/CD18的表达,干预中性粒细胞黏附。
Objective To observe the expression of adhesion molecular CD11b/CD18 in peripheral neutrophils and its relation with arteriosclerotic obliterans （ASO）, and to study the effect of Xuefu Zhuyu Oral Liquid （XZOL） on it. Methods Flow cytometery analysis was used to detect the expression of CD11b/CD18 in peripheral neutrophils of 30 patients with ASO and 30 healthy subjects by direct immunofluroscent technique. Neutrophils were separated from whole blood of ASO patients and cultured, CD11b/CD18 were detected after the cultured cells were interfered with XZOL dilution at different time points （1h,6h, 12h）, Results The expression of CD11b/CD18 in neutrophils in ASO patients was significantly higher than that in the healthy subjects （P 〈 0.05） and stepped in keeping with the severity of the disease. It was significantly lowered in the treated group 6 and 12 h after XZOL intervention, showing significant difference as compared with that in the control group and the level 1 h after medication （P〈0.05）. Conclusion CD11b/CD18 may involve in the pathogenesis of AS（3 and be related to the severity of arteriosclerosis. The possible mechanism of XZOL in treating and preventing ASO might be through reducing the expression of CD11b/CD18 in peripheral neutrophils to interfere the adhesive function of them.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2006年第2期125-127,共3页
Chinese Journal of Integrated Traditional and Western Medicine
