摘要
目的:观察脑灵汤对A lzhe im er病(AD)模型大鼠行为学及脑内海马部位β-淀粉样前体蛋白(APP)mRNA影响。方法:以D-半乳糖腹部注射合并铝盐皮下注射制作AD大鼠模型,行γ-电迷宫实验检测学习记忆能力及RT-PCR法观察大鼠脑内海马APPmRNA的变化。结果:脑灵汤治疗30 d后大鼠学习记忆能力较模型组和对照组明显增强(P<0.05);大鼠脑内海马部位APP基因mRNA表达的水平亦较模型组和对照组明显降低(P<0.05)。结论:脑灵汤抑制和下调APPmRNA表达的水平,减少海马结构中β-淀粉样蛋白的沉积,是改善模型大鼠学习记忆的作用机制之一。
Objective To observe the effect of naoling decoction （NLD） on the behavior and the mRNA expression of β-amyloid precursor protein （APP） in the hippocampus in rat model with Alzheimer＇s disease （AD）. Methods D-galactose was intra-abdominally injected and AICl3 was hypodermically injected to build the AD models. The behavior of rats was measured by γ-electric maze and the level of APP mRNA in the model rat hippocampus was observed by RT-PCR. Results . The learning and memory capacity in the NLD group was improved and the APP mRNA expression level was significantly lower in the NLD group compared with the untreated model and the control group （ All P 〈 0.05 ）. Conclusion NLD can lower the expression of APP mRNA to reduce β-amyloid protein deposition of rat hippocampus, which may be one of the mechanisms of improving the learning and memory capacity of AD model rats.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2006年第1期60-62,共3页
Journal of Central South University ：Medical Science
