摘要
目的探讨^(125)Ⅰ粒子植入对荷人肝癌裸鼠移植瘤生长和细胞增殖及凋亡相关蛋白的影响。方法建立12只荷人肝癌 BEL-7402裸鼠模型,治疗组和对照组各6只。治疗组每只裸鼠移植瘤内植入1枚活度为33.3 MBq 的^(125)Ⅰ粒子,对照组不进行干预。治疗后每3～4 d 测量1次肿瘤直径,并计算治疗组肿瘤的体积抑制率。21 d 后处死卡果鼠,制作肿瘤组织标本,进行常规病理学检查和免疫组织化学检测增殖细胞核抗原(PCNA)、bcl-2和 bax 的表达。结果 ^(125)Ⅰ粒子治疗组肿瘤体积最大抑制率为49.2%,病理检查显示近粒子处肿瘤细胞变性坏死,远离粒子处可见存活肿瘤细胞。免疫组织化学显示治疗组 PCNA 表达强度和 bcl-2/bax 比值均低于对照组。结论 ^(125)Ⅰ粒子植入低剂量持续照射可直接杀死荷瘤鼠肝癌细胞或抑制肿瘤生长。
Objective To explore ihe-effect and-i-nfl-ue-nce of tumor growth, cell proliferation and apoptosis related protein of ^125I seed brachytherapy in human hepatocellular carcinoma（ HHC ） bearing nude mice. Methods Twelve HHC BEL-7402 cells bearing nude mice were equally divided into treated and control group. One ^125I seed with 33.3 MBq was implanted into each mouse in treated group. Afterward the dimensions of tumor in both groups were measured every 3 ～ 4 d and also the tumor volume inhibition rates were calculated. Twenty-one days later, nude mice sacrificed and routine pathological examination proceeded. The expression of proliferating cell nuclear antigen （PCNA）, bcl-2 and bax were detected by immunohistochemical methods. Results The maximum tumor inhibition rate of treated group was 49.2%. Routine pathological examination found degenerative necrosis at the site was nearby the seed but the alive tumor cell still presented faraway from the seed. The expression of PCNA and the ratio of bol-2/bax in treated group were lower than those in control group. Conchmion The sustained irradiation of ^125I seed brachytherapy can direct kill the HHC cell in nude mice or inhibit the proliferation of tumor cells.
出处
《中华核医学杂志》
CAS
CSCD
北大核心
2006年第1期23-25,共3页
Chinese Journal of Nuclear Medicine
