摘要
目的:建立成人成牙本质细胞原位培养模型。方法:取20～30岁成人新鲜、健康、完整的第三磨牙30颗,随机分为拔髓实验组、有血清培养组和无血清培养组,每组各10颗。冠根分离,拔髓,将成牙本质细胞保留在牙冠上,进行有血清和无血清的原位培养,每天换液,连续培养7d。通过光镜检测细胞保留情况,扫描电镜检测细胞形态和分布特征,台盼蓝检测细胞活性,对模型进行鉴定。结果:室温拔髓后,成牙本质细胞保留在髓室壁上,在7d的培养过程中细胞有活性,形态保持良好。结论:采用有血清和无血清培养,均可建立成人成牙本质细胞培养模型。
PURPOSE： To establish an in situ culture model of adult odontoblasts. METHODS： Thirty intact and healthy third molars freshly prepared from 20-30 year old individuals were randomly divided into three groups.Each group had 10 molars.Group 1 was pulp tissue extraction group.Group 2 was serum-containing culture group.Group 3 was serum-free culture group. The root was dissected from the crown and the pulp was pulled out to make odontoblasts remaining in the crown. The odontoblasts were cultured in situ either in medium containing serum or serum-free medium for up to 7 days. The growth status of the cells was examined by light microscopy and cell morphology and distribution was analyzed by scanning electron microscopy. Cell viability was determined by trypan blue staining. RESULTS： After pulp removal at room temperature, odontoblasts remained in the wall of the pulp chamber, and kept viable and good morphology during the 7-day culture. CONCLUSION： We have successfully established an in situ culture model of adult primary odontoblasts in either serum-containing or serum-free medium.
出处
《上海口腔医学》
CAS
CSCD
2006年第1期88-92,共5页
Shanghai Journal of Stomatology
关键词
成牙本质细胞
拔髓实验
有血清培养
无血清培养
原位培养
Odontoblast
Tissue extraction
Serum-containing culture
Serum-free culture
Culture in situ
