摘要
以牛皮胶原成分为底物，研究了胃蛋白酶用量和温度对胶原成分水解动力学的影响。实验结果表明，在15～40℃范围内，提高温度有利于水解过程的进行；提高酶用量，也可以促进水解过程。胃蛋白酶对牛皮胶原成分的水解过程基本符合内切酶限制水解历程，其动力学模型为：C（-NH2）=b^-1，1n[1＋a·b·t]，式中动力学参数“随着实验条件的不同而不同，其与实验条件的关系有待进一步的研究，而动力学参数b为胃蛋白酶住一定条件下水解牛皮胶原成分的水解动力学常数，其值在6．0附近波动。
The effects of pepsin offer and temperature on hydrolysis kinetic of calf collagen components were investigated. The results indicated that in the range 15-40℃, the hydrolysis process was facilitated by the increase of enzyme offer and temperature. Meanwhile, the hydrolysis mechanism the course of limited hydrolysis of inner-enzyme, and the dynamic model of hydrolysis can be expressed as： C （-NH2）=b^-1 . 1n[1＋a·b·t]. Where the dynamic parameter a varies with the conditions of hydrolysis and the relation between value a and hydrolysis-conditions needs to be further investigated. The dynamic parameter b, the value around 6.0, is a hydrolysis dynamic constant of calf collagen components by pepsin.
出处
《皮革科学与工程》
CAS
2006年第1期29-32,共4页
Leather Science and Engineering
基金
国家"863"计划项目
2003AA647020
关键词
酶
胶原
水解
动力学
collagen
enzyme
hydrolysis
dynamic
