摘要
背景与目的：研究pEgr-TNFα重组质粒在稳定转染的食管癌细胞中的辐射诱导表达，及其联合放射治疗抑制食管癌细胞生长的效果。材料与方法：将脂质体包裹的pEgr-TNFα重组质粒，转染食管癌细胞系EC9706中，经G418筛选，获得稳定表达的细胞；采用ELISA方法检测0．075Gy和2Gy剂量x射线诱导后TNFα的表达；观察该工种剂量X射线照射后，EC9706细胞的增长情况。结果：pEgr-TNFα重组质粒转染EC9706细胞，并获得稳定转染的细胞；大剂量x射线照射和低剂量X射线照射均可诱导TNFα表达增强，为对照组的6～6．3倍（P〈0．01）；稳定转染的细胞经0．075Gy和2GyX射线照射，8d后细胞数明显低于未转染细胞组（P〈0．01）和稳定转染的假照射组（P〈0．01）。结论：体外pEgr-TNFα基因．放射联合治疗有明显的抑制食管癌细胞生长的作用。
BACKGROUND ＆ AIM： To study the expression of pEgr-TNFα induced by ionizing irradiation in stably transfected esophageal cancer cells and to study the inhibition effects of pEgr-TNFα combined with ionizing irradiation on esophageal cancer cells. MATERIAL AND METHODS： Esophageal cancer cell line EC9706 was transfected with pEgr-TNFα plasmids and selected through G418 to get stable expression cells. TNFα expression was detected by ELISA. The inhibition effect of pEgr-TNFα was observed after different doses of X-ray irradiation, rESULTS： pEgr-TNFα plasmids were transfected into EC9706 cells with stable expression. High dose and low dose X-ray irradiation could all enhance the expression of TNFα, about 6-6.3 times of control group （P〈O.001） . Ceils stably transfected was given 0.075 Gy and 2 Gy X-ray irradiation, number of cells was less than non-transfected group and sham group （P 〈0.01- 0.001） 8 d later. CONCLUSION： pEgr-TNFα gene-radiotherapy can significantly inhibit the growth of esophageal cancer cells in vitro.
出处
《癌变．畸变．突变》
CAS
CSCD
2006年第1期6-8,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金资助项目(No.30210103904)
广东省科技计划项目资助(No.2003C30304)
