摘要
本文研究了PP333与BA组合对怀地黄(Rehmanniaglutinosa)试管苗生长发育的影响。结果表明:(1)与对照相比,不同浓度的PP333与0.5mg.L-1BA组合均严重地抑制试管苗根、茎和叶的生长,表现为植株矮小,生根晚,前期根畸形生长(呈棒状或球状);(2)与单独使用0.5mg.L-1BA相比,低浓度PP333(0.01和0.1mg.L-1)与0.5mg.L-1BA组合促进茎的伸长和加粗;(3)2mg.L-1PP333与0.5mg.L-1BA组合能显著地提高愈伤组织芽的分化率和腋芽的萌发率,有利于试管苗的快速繁殖。
The influence of PP333 with benzylaminopurine （BA） on growth of Rehmannia glutinosa plantlets was studied in this paper. Combinations of （0.5 mg·L^-1） BA and different concentrations of PP333 inhibited the growth of roots, stems and leaves significantly. Plantlets were short and small, and roots appeared later. The roots looked like clubs or balls initially. Compared with （0.5 mg·L^-1） BA, （0.5 mg·L^-1） BA with lower concentrations of PP333, at 0.01 or 0.1 mg·L^-1, improved the extension and thickness of stems. MS medium supplied with （2 mg·L^-1） PP333 and （0.5 mg·L^-1） BA increased the rate of bud formation of callus and pullulation of axillary buds observably, which is good for largescale in vitro propagation.
出处
《植物学通报》
CSCD
北大核心
2006年第1期56-59,共4页
Chinese Bulletin of Botany
基金
河南省重点科技攻关项目(0423032100)
