摘要
将病毒保护剂运用于猪瘟兔化弱毒牛睾丸细胞疫苗生产中,结果表明:20%的病毒保护剂对产毒有轻微损伤作用,10%的病毒保护剂要略好于对照组,通过蛋白浓度测定证实提高22%。试验组和对照组的反复冻融试验表明对照组冻融六次即为临界点,病毒保护剂组达10次。冷冻保存期试验表明对照组保存三个月合格,而病毒保护剂组可达五个月。37℃耐热试验表明对照组在8 h内合格,病毒保护剂组达10 h。细胞毒电镜观察试验及主要保护性抗原基因E2、E0测序比对结果表明,病毒保护剂不改变病毒粒子形态,且不产生基因突变。抗体水平和免疫攻毒试验结果表明,病毒保护剂能刺激机体早期抗体的产生,且免疫保护力坚强。
Applying virus preserver into bovine testicle cell vaccine production of HCLV,we found virus preserver brought about light lesion while adding 20 % and brought about good result while adding 10 %,however the proliferation conclusion need to be reconfirmed .As the result of freezing and thawing again and again showed ,6 times by repeating freezing and thawing in the control was critical point .If it was over 6, the control would not be eligible whereas the experiment would be eligible exceeding 10 times. Cryopreservation for a long time showed that control would be eligible in 3 months while experiment would be eligible in 5 months.The result bearing heat showed control would be eligible in 8 hours at 37 ℃,while experiment would be eligible in 10 hours at 37 ℃ .The result of the observation by electron microscope and major protective antigen gene E2 and E0 sequencing blast showed that virus preserver did not effect HCLG virus morphology and did not produce gene mutation.The results about immunity antibody levels detecting and innoclating CSFV showed, virus preserver could stimulate primal antibdy appearance, and retain strong immunity.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2006年第1期16-21,共6页
Chinese Journal of Preventive Veterinary Medicine
