摘要
以盆栽草莓为材料,研究了叶面喷布5-氨基乙酰丙酸(ALA)对草莓植株光合作用、叶绿素荧光特性、抗氧化酶活性和丙二醛(M DA)含量的影响.结果表明,100 m g/L ALA处理显著提高草莓叶片净光合速率(Pn),而且这一效应可能与其促进叶绿素含量和羧化效率(CE)提高,降低光呼吸速率(Rp)有关.叶绿素荧光动力学资料显示,ALA处理降低高光强(1 500μm o l.m-2.-s 1)下草莓叶片的初始荧光(Fo),表明它对光合膜系统有一定保护作用.ALA处理不仅明显提高草莓叶片最大荧光(Fm)和可变荧光(Fv),而且提高PSⅡ实际光化学效率(ΦPSⅡ)、光化学荧光猝灭(qP)、非光化学荧光猝灭系数(N PQ)、表观光合电子传递速率(ETR)、光化学速率(P CR)和天线热耗散(D),而降低了光下相对光合限制值(L(PFD)),表明叶绿素荧光产额提高和天线热耗散是保护光合器官并提高光合效率的两个方面.叶片抗氧化酶活性测定以及超氧化物歧化酶(SOD)活性抑制剂二乙基二硫代氨基甲酸(DDC)处理结果表明,ALA对草莓光合作用的促进作用还与其提高抗氧化酶活性有关.
Pot-planted strawberry was used to study the effects of ALA topdressing on the photosynthesis, chlorphyll fluorescent properties and anti-oxidant enzyme activities and MDA contents of strawberry plants. The results indicated that ALA topdressing at 100 mg/L significantly increased the net photosynthetic rate of strawberry leaves and that the increasing effect probably had something to do with its increasing effect on chlorophyll content and carboxylation efficiency and lowering photorespiration. The ki- netic data of chlorophyll fluoresecence revealed that ALA topdressing reduced the non-variable fluorescence （F,,） of strawberry leaves at high light intensity （1500μmol·m^-2·s^-1）,which indicated that ALA has certain protecting effect on photosynthetic membrane system. ALA topdressing could not only remarkably raise the maximum fluorescence （Fm） and variable fluorescence （Fτ） of strawberry leaves but also increase the actual photochemical efficiency of PSⅡ（φPSⅡ）,photochemical quenching （qP） and non-photochemical quenching （NPQ）,electron transport rate （ETR）,photochemical rate （PCR）,and antennae heat dissipation rate, thereby lowering the PFD limitation,and this showed that chlorophyll fluorescence yield and antennae heat dissipation were two respects in protecting photosynthetic organs and improving photosynthetic efficiency. The measurements of anti-oxidant enzymes and the treatment results of diethyldithiocarbamate （DDC）,the inhibitor to SOD activity,revealed that the enhancing effect of ALA on strawberry photosynthesis had something to do with its improving effect on the activities of other anti-oxidant enzymes.
出处
《西北植物学报》
CAS
CSCD
北大核心
2006年第1期57-62,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金项目(30471181)
