摘要
目的应用中药血清药理学的方法，通过时间一效应关系研究，探讨大补阴丸（汤）含药血清对CJ（Campylobacter jejuni，空肠弯曲菌）致敏的自身免疫病模型小鼠（CJ小鼠）T、B淋巴细胞活性的影响及其时间-效应关系。方法将健康雌性ICR小鼠随机分为正常对照组（normalcontrolgroup，N）、模型组（modelgroup，M）、模型对照组（model control group，MC）以及5个时相给药组（30min、1h、1．5h、2h、3h），共8组。除正常对照组外，其他各组均用空肠弯曲菌免疫小鼠制备自身免疫病模型。从造模第15d起，开始以大补阴丸（汤）（0．4g生药饮片／ml）给不同时相用药组小鼠灌胃，连续用药10d。并分别于第10次给药后30min、1h、1．5h、2h、3h取血，分离血清；同时也分别取N组、MC组血，分离血清。继之处死小鼠，称量脾，计算脾指数。将上述血清与M组小鼠的脾细胞体外培养。检测DBYW含药血清对T、B淋巴细胞活性的影响，并绘出时-效关系变化曲线。结果大补阴丸（汤）在1～1．5h时相含药血清，可明显抑制CJ小鼠的T、B淋巴细胞增殖活性，降低CJ小鼠体内异常升高的ds-DNA、ss-DNA抗体水平和脾指数，改善其因自身免疫反应所致的肝肾病理损害。结论大补阴丸（汤）含药血清对异常免疫机能状态下的T、B淋巴细胞活性具有明显的免疫抑制作用，且在1～1．5h时作用最明显。
Objective To explore the effect of Da-Bu-Yin Wan（DBYW） on proliferation of T ＆ B lymphocytes in autoimmune mice induced by Campylobacter Jejuni with a method of serum-pharmacology study. Methods Healthy female ICR mice were divided into 8 groups at random as normal control group（N）, model group（M）, model control group （MC） and administering group （administration interval ： 30min、 1 h、 1.5 h、 2 h、 3 h）. The mice were induced by Campylobacter Jejuni with Complete Freund＇ s Adjuvant as autoimmune animal model except N group. From the fifteenth day after primary immunization, the administering group were given DBYW through gastric route in a dosage of 0.4 g/ml while MC group were given physiological saline. All the administration lasted for 10 days. After the last administration, blood samples were taken in 30min, 1 h, 1.5 h,2 h and 3 h respectively to detect the proliferation of T ＆ B lymphocytes. The curve of time-effect relationship was marked to show the drug efficacy. Results It was found that the proliferation of T ＆ B lymphocytes were markedly inhibited by the drug-containing serum taken at 1 - 1.5 hrs after the treatment （P 〈 0.01）. Conclusion The drug-containing serum of mice taken at the time of 1- 1.5 hrs after ingestion of DBYW could regulate the proliferation of T＆B lymphocytes in autoimmune mice induced by Campylobacter Jejuni.
出处
《皖南医学院学报》
CAS
2006年第1期7-11,共5页
Journal of Wannan Medical College
基金
安徽省教育厅自然科学研究基金项目(2002kj301)
关键词
大补阴丸(汤)
空肠弯曲菌
血清药理学
时效关系
淋巴细胞活性
DaBuYinWan（Tang ）
campylobacter jejuni
serum pharmacology
time-effect relations
lymphocytes activation
