摘要
目的观察过热、过咸饮食引起胃黏膜损伤以致造成胃黏膜萎缩的过程中一氧化氮(NO)及一氧化氮合酶(NOS)的动态变化。以及在该过程中采用抗氧化荆干预胃组织和血清中氧化损伤重要指标丙二醛(MDA)的动态变化。方法采用55℃、1．5％NaCl 对实验大鼠连续灌胃每日1次,对照组采用等量的25℃水灌胃。在灌胃期间每隔4周处死一批大鼠。将大鼠麻醉后腹主动脉取血分离血清,同时摘取胃组织,制成组织匀浆,分别采用试剂盒测定血清和胃组织中NO和NOS。采用Lowry’s法测定组织匀浆中总蛋白的含量。结果实验动物血清中NO和NOS活性随着大鼠周龄的增加而升高,以热盐水组升高显著。胃黏膜组织中NO和NOS活性随着时间的延长逐渐升高,而12周后,随着时间的延长,表现出了下降的趋势。结论提示热盐水所致萎缩性胃炎的发病过程中NO在其中发挥着重要作用。
Objective To investigate the dynamic variation of Nitric oxide （NO） and nitric oxide synthase（NOS） in the process of atrophic gastritis formation caused by over salt hot diet. Methods The atrophic gastritis rats were made by affusing over salt hot water ,and the rats were killed group by group every 4 weeks . The NO and NOS in serum and gastric mucosa of atrophic gastritis rat were detected. Results The NO and NOS in rat＇s serum increased with the age of rat prolonged ,especially in hot salt water group. The activity Of NO and NOS in gastric mueosa increased with the time prolonged but decreased after 12 weeks . Conclusion NO play an important effect in the formation of atrophic gastritis.
出处
《临床消化病杂志》
2006年第1期42-44,共3页
Chinese Journal of Clinical Gastroenterology
基金
西安市科技攻关计划(GG04117)
关键词
萎缩性胃炎
热盐水
一氧化氮
一氧化氮合酶
Chronic atrophic gastritis
High salt hot water
Nitric oxide
Nitric xoide synthase
