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FISH技术在废水处理中的应用 被引量：2

Fluorescence in Situ Hybridization for the Use of Wastewater Treatment
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摘要 该文介绍了FISH(Fluorescence in situ hybridization)技术与DGGE/TGGE、PCR扩增技术、共聚焦激光扫描显微镜以及生物传感器等结合使用,可直观形象的研究微生物在废水处理系统中的形态、生理变化,以及微生物种群的演替的规律;为筛选和驯化活性污泥或生物膜中的对废水处理起主导作用的微生物,提供更加有效、快速、可视的信息,促进废水处理技术的发展。 The application ofFISH combined with DGGE/TGGE, PCR, confocal laser scanning microscopy（CLSM）, micro-sensor and so on, could investigate morphology of microorganisms, changes of microbial physiology and discipline of microbial succession in bioreactor system intuitively and iconicaUy. Meanwhile, it could provide more efficient, instant and visible information for sieving and culturing the activated sludge or bio-films in disposing wastewater, and accelerate the development of wastewater treatment technology.
作者 董远湘 李小明 尹疆 杨麒 金科 钟琼
机构地区 湖南大学环境科学与工程系 湖南省环境保护科学研究所
出处 《生物技术》 CAS CSCD 2005年第6期94-96,共3页 Biotechnology
基金 国家自然科学基金资助项目(50748054) 国际科技合作重点项目(2003AA649020)
关键词 FLSH 活性污泥 生物膜 分子生态学 FLSH activated sludge bio- films molecular ecology
分类号 Q789 [生物学—分子生物学]
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参考文献19

  • 1Van Hulle SWH,Van Den Broeck S,Maertens J,etal.Practical experiences with start-up and operation of a continuously aerated lab-scale SHARON reactor//Communications in Applied Biological Sciences[C].Gent,Belgium:Proceedings FAB Symposium,2003,77-84.
  • 2Van Hulle S WH,Volcke EI P,Lopez Teruel J.Influence of temperature and pH an the kinetics of the SHARON nitritation process//Proceedings 4th World Water Congress and Exhibition[C].Marrakech,Marokko,2004,88-89.
  • 3Van Kempen R,Mulder J W,Uijterlinde C A,et al.Full scale experience of the SHARON process for treatment of rejection water of digested sludge dewatering[J].Water Science Technology,2001,44(1):145-152.
  • 4Van Hulle S W H,Van Den Broeck S,Maertens J,et al.Construction,start-up and operation of a continuously aerated laboratory-scale SHARON reactor in view of coupling with an Anammox reactor[J].Water SA,2005,31:327-334.
  • 5Peter A Wilderer,Hans-Joachim Bungartz,Hilde Lemmer,et al.Modern scientific methods and their pothential in wastewater science and technology[J].Water Research,2002,36:370-393.
  • 6高平平,赵立平.可用于微生物群落分子生态学研究的活性污泥总DNA提取方法研究[J].生态学报,2002,22(11):2015-2019. 被引量：77
  • 7Hemant J Purohit,Atya Kapley,Aditi A.Moha-rikar,et al.A novel approach for extraction of PCR compatible DNA from activated sludge samples collected from different biological effiuent treatment plants[J].Journal of Microbiol-ogical Method,2003,52:315-323.
  • 8Futoshi Kurisu,Hirogasu Satoh,Takashi Mino,et al.Microbial community analysis of thermophilic contact oxidation process by using ribosomal RNA approaches and the quinone method[J].Water Resarch,2002,36:429-438.
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生物技术
2005年 第6期

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