摘要
目的观察经颅磁刺激(TMS)对大鼠大脑中动脉栓塞后神经功能恢复和健侧突触结构参数变化的影响,探讨其代偿机理。方法24只雄性SD大鼠应用线栓法建立大鼠大脑中动脉栓塞(MCAO)模型,随机分为TMS组和对照组,前者给予TMS治疗28d,后者常规饲养,观察大鼠神经功能恢复情况和健侧大脑感觉运动皮质区突触超微结构参数的变化。结果TMS组大鼠治疗后神经功能恢复优于对照组(P<0.05),电镜下观察到TMS组大鼠脑感觉运动皮质区突触界面曲率、突触后致密物质(PSD)厚度增加,突触间隙变窄,与对照组比较,差异有统计学意义(P<0.05)。结论TMS可促进脑缺血大鼠神经功能的改善,其机制可能与健侧突触超微结构参数的改变有关。
Objective To evaluate the effects of TMS on the brain plasticity and functional outcome after cerebral infarction in rats. Methods Twenty-four male Sprague-Dawley rats were divided randomly into a model group and a TMS group. The rat models of focal cerebral infarction were established with unilateral middle cerebral artery （MCA） suture occlusion method. The rats of TMS group were given additional 4 weeks of TMS treatment commenced at I day after infarction （2 times per day, 30 pulses per time） , while those in the control group were reared in their original living state. Synaptic substructure in the sensori-motor cortex area was assessed morphologically and quantitatively. Results When compared with the model group, the rats in the experimental group had a significant improvement in terms of their neural functions （P 〈 0.05 ）. Quantitative electron microscopy showed that the synaptic curvatures and post-synaptic density （PSD） were significantly increased, and that the synapse cleft width was narro- wer in the TMS group as compared with those of the model group （P 〈 0.05 ）. Conclusion The improvement of neural function in the rats with TMS were likely to be related to the changes of synaptic substructure and the increased synaptic plasticity.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2005年第12期707-710,共4页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
脑缺血
经颅磁刺激
突触结构参数
大鼠
Cerebral ischemia
Transcranial magnetic stimulation
Synaptic substructures
Rat
