摘要
目的使用F eeney法建立符合临床研究的大鼠闭合性脑损伤模型,并对其进行评估。方法应用自行设计制造的撞击装置,按照F eeney法复制闭合性脑损伤。撞击装置由支架、套管、砝码和脚板四部分组成。采用96只3月龄SD雌性大鼠随机分为3组:对照组(A组),不给予撞击;轻伤组(B组),打击能量400 g.cm撞击一次;重伤组(C组),打击能量800 g.cm撞击一次;每组32只。分别于致伤后6、24 h,3、7 d,观察大鼠脑损伤早期生理反应、病理改变,并测定脑含水量的变化。结果B、C组大鼠伤后均表现为血压骤升后骤降、脉搏加快、呼吸深快或暂停,甚至永久停止;尤以C组明显。B、C组致伤后6 h均出现损伤灶皮质挫伤淤血、周围神经元变性等病理改变,脑含水量明显增加,7 d后大致正常;伤后12 h损伤灶有透明血栓形成,3 d伤灶周围神经胶质细胞增生,7 d伤灶局部液化坏死。而且病理损伤程度随撞击力和撞击面积上升而逐渐加重,当撞击力上升至1 200 g.cm时,大鼠多因呼吸骤停死亡。结论建立的大鼠颅脑损伤模型基本符合临床闭合性脑损伤的病理学改变与病理生理特点,是一种较为实用的方法。
Objective To set up and to evaluate an acute closed brain injury model in rats. Methods The acute closed brain injury was produced in rats by using an impactor consisting of a stand, a guide tube, a weight and a footplate. Ninety-six SD rats were divided into a control group（n 32, no impact）, a mild injury group（n=32, impact once at force level of 400 g· cm） and a severe injury group（n=32, impact once at force level of 800 g· cm） to elucidate the physiological responses, the patbopbysiological changes and brain edema after brain injury at different injury levels. Results In the mild injury group and the severe injury group, a sudden rise or reduction of blood pressure, deep and fast breath apnea, and pain reflects inhibition were observed. The responses were more obvious in the severe injury group than in the mild injury group. The water content of the brain increased after 6 hours of injury. The pathological contusion and edema of brain were noted or above the impact force level of 800g. cm. When the impact force rose to or over 1200g·cm, the animals died of persistent apnea mostly. Conclusion Although the established closed brain injury model with different biomecbanical mechanisms as the clinical brain injury, it is in conformity with pathological changes and patbopbysiological characteristics of acute clinical brain injury, it can be utilized extensively because of its convenient and practice.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2005年第12期1015-1018,共4页
Chinese Journal of Reparative and Reconstructive Surgery
关键词
闭合性脑损伤
脑水肿
评估
大鼠
Closed brain injury Brain edema Evaluation Rat
