摘要
目的：建立通光藤中C21甾体糖苷成分的含量测定方法，为通光藤药材的质量控制提供依据。方法：样品用甲醇提取，经ADS-7大孔吸附树脂纯化，以通光藤苷-H为对照品，浓硫酸-甲醇（4：1）显色，在325nm处测定通光藤中C2l甾体糖苷含量。结果：在10，6～148，4μg吸光度与含量成良好线性关系，r=0．9999（n=6），3个含量的加样回收率分别为95．4％，97．7％，96．9％，RSD〈2．4％（n=5）。结论：本法操作简便、快速、灵敏、重复性好，可以作为通光藤药材质量评价的定量分析方法。
Objective： To establish a quantitative method to deter mine C21 steroidal glycosides in Marsdenia tenacissima. Method： Methanol was used as the extraction solvent and the samples were purified by macroporons resin ADS-7. A mixture of sulfuric acid-methanol （4：1 ）was used as color-producing reagent. The absorbance was measured at 325 nm. Result： There is a good linearity （ r = 0.999 9, n = 6） within the range of 10.6-148.4/μg. The average recoveries of tenacissoside-H at different concentrations were 95.8% to 97.1% with RSD less than 2.4% （ n = 5）. Conduslon： This method is reliable as a quantitative analytical method for the quality assessment of M. tenacissima.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2005年第22期1745-1748,共4页
China Journal of Chinese Materia Medica
关键词
通光藤
C21
甾体糖苷
通光藤苷-H
比色法
Marsdenia tenacissima
C21 steroidal glycosides
tenacissoside-H
colonmetric method
