摘要
在体外用Ｃｕ^（２＋）诱导法使ＬＤＬ氧化修饰，利用紫外法监测氧化过程中所形成的结合二烯键，发现整个反应分为３个时相，即延迟期、快速反应期和分解期。利用硫代巴比妥反应物测定，发现氧化后ＬＤＬ中每ｍｇ蛋白脂质过氧化物增加了１５倍。
Low density lipoprotein(LDL)was oxidized with Cu￣(2+)in vitro.A lag phase was followed by oxidave modification after addition of CuCl_2, and about 15 h,the reaction reach a peak. LDL oxidation was continuously monitored spectrophotometrically at 234 nm to follow the formation of conjugated dienes.Three phases were found in oxidation curve:Lag phase, propagation phase and decomposition phase. By determinating thiobarbituric acid reaction substances(TBARS),we found the lipid peroxidation increased as much as 15 times in LDL.
关键词
低密度脂蛋白
氧化修饰
制备
low density lipoprotein
oxidative modification
conjugated dienes
thiobarbituric acid reaction substances(TBARS)
