秋水仙碱对大鼠内毒素性休克的影响

Effect of colchicine on endotoxic shock in the rat

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摘要静脉内注射脂多糖（ＬＰＳ）６ｍｇ／ｋｇ可引起大鼠血清肿瘤坏死因子－α（ｔｕｍｏｒｎｅｃｒｏｓｉｓｋｃｔｏｒ－α，ＴＮＦ－α）短时间增多和高甘油三酯血症，同时出现血压下降、心输出量减少、四肢冰凉等休克症状，动物大多于１２小时内死亡。提前１小时腹腔内注射秋水仙碱（ｃｏｌｃｈｃｉｎｅ，Ｃｏｌ．）０．５ｍｇ／ｋｇ可使ＬＰＳ诱导的血清ＴＮＦ－α水平升高的程度明显减轻；同时，血压维持正常，心输出量显著高于单独注射ＬＰＳ组，血清甘油三酯水平正常。本文再次证实ＴＮＦ－α在内毒素性休克中的重要作用，而Ｃｏｌ．减轻休克症状的机制很可能与其抑制ＴＮＦ－ａ的产生密切相关。 Intmvenous injection of lipopolysaccharide(LPS, 6 mg/kg)in the rat resulted in anabrupt,monophasic but tiansient elevation of the serum TNF-α level followed by a gradual in-crease in serum triglyceride co ncentration within a period o f 4 hours. Changes in these serum pamrneters were associated with shock symptoim including a dramatic fall of arterial blood pres-sure and cardiac output,cold extremities etc. majority of the animals died within 12 hours. Intraperitoneal injection of colchicine(Col., 0.5mg/kg)one hour before LPS administration brought about a marked alleviation ofthe LPS-induced elevation of serum TNF-α level. Besides, ar-terial blood pressure and serum triglyceride concertration were maintained within the normal limits in the Col. -pretreated rodents. The cardiac output,however,was apparently higher than thatin animals treated with LPS alone. The present work once more confirmed the important role of TNF-αin endotoxic shock and the mechanism underlying the relief of shock symptoms by Col. -pretreatment might be closely related to its iuhibition TNF-αproduction.