摘要
目的:了解壳聚糖对自制磷酸钙骨水泥(CPC)物理性能、超微结构及生物相容性的影响.方法:分别以50g/L乳酸壳聚糖和1mol/L磷酸氢二钠为液相制备CPC试样,通过防水试验、测定初步凝结时间、抗压强度;扫描电镜观察凝固体超微形态;大鼠肌肉植入及骨髓基质干细胞表面种植试验比较两者差异.结果:复合壳聚糖的CPC在水中更稳定,混合后即刻投入生理盐水3min内不散开;平均初步凝结时间8.38min,对照组23.68min;平均抗压强度6.52MPa;对照组2.08MPa.扫描电镜发现凝固体表面为颗粒状及片状晶体,实验组晶体互相连接,孔隙较对照组少.植入肌袋1wk有轻微炎症反应,4wk后反应减退,12wk后实验组材料变软,对照组外形基本未变.肌肉无坏死.电镜下细胞可在水泥盘上贴附、生长,实验组可见的细胞数较多.结论:CPC-壳聚糖复合物在潮湿条件下更稳定,固化时间缩短,抗压强度提高,生物相容性更好.
AIM： To investigate the effect of chitosan on the physical characteristics, ultramicrostructure and biocompatibility of a self-made calcium phosphate cement （CPC）. METHODS： Two different cement formulations were prepared by mixing CPC powder respectively with 50 g/L chitosan lactate and 1 mol/L dibasic sodium phosphate used as liquid phase, For physical properties, washout resistance, primary setting time and compressive strength were measured. Scanning electron microscopy （SEM） was used to examine the microstructure and the intramuscular implantation of a rat model and rat mesenchymal stem cells （ RM- SC） seeded on CPC disks were used to compare the biocompatibility of the two formulations. RESULTS： CPC-chitosan composite was more stable in water than conventional CPC and it did not disintegrate within 3 min even when placed in water immediately after the mixing. The mean setting time was 8. 38 min in CPC-chitosan and 23. 68 min in the control. The mean compressive strength of composite increased to 6.52 MPa, whereas the control samples exhibited much lower values （2.08 MPa）. SEM examinations showed that globular and platy particles were formed on surfaces of all samples but the particles on the surface of the CPC-chitosan composite seemed to connect together viscously, with smaller pores than those of control. Slight signs of inflammation, which disappeared after four weeks, were detectable around the specimens implanted within one week. After 12 weeks, the experimental implants became soft, while the controls remained the same in appearance. Necrosis was not detected in the muscles around the implants. SEM showed that cells adhered to CPC-chitosan composite disks, but the visible amount of the adherent cells was greater than that of control. CONCLUSION： Chitosan is suitable for the stabilization of CPC in a wet environment for it accelerates the setting reaction and improves the mechanical properties and biocompatibility of the cements.
出处
《第四军医大学学报》
CAS
北大核心
2005年第21期1981-1984,共4页
Journal of the Fourth Military Medical University
基金
国家自然科学基金(30330610)
关键词
磷酸钙骨水泥
壳聚糖
抗压强度
生物相容性
calcium phosphate cement
chitosan
compressive strength
biocompatibility
