摘要
目的:探讨大鼠经腹心包腔内注射术的可行性、安全性、有效性及其在心脏疾病研究中的应用价值。方法:采用经腹心包腔内注射术,分别将染料、携带绿色荧光蛋白基因的重组相关腺病毒溶液和无菌等渗盐水注入相应组别大鼠心包腔内,然后分别进行尸检、血流动力学检测和冰冻切片荧光显微镜检查。结果:染料组大鼠尸检证明全部注射成功;绿色荧光蛋白组大鼠心肌组织在荧光显微镜下均发出弥漫的绿色荧光;绿色荧光蛋白组和等渗盐水组大鼠术中及术后无死亡,其血流动力学指标与正常对照组大鼠无差异。结论:经腹心包腔内注射术具有操作简单、可行性强、安全有效和适合实验小动物等优点,为研究心脏疾病提供了一种简单、有效、安全、经济的方法。
Objective：To study the feasibility, safety and effectiveness of intrapericardial injection with a trans-diaphragmatic approach in rats and its value in the study of heart diseases. Methods： Blue-black ink, recombinant adeno-associated virus carrying enhanced green fluorescent protein （eGFP） gene, 0.9% sodium chloride solution were respectively injected into pericardium of rats in three different groups （Group ink, Group eGFP and Group sodium chloride） by intrapericardial injection with a trans-diaphragmatic approach. Autopsy was done and hemodynamic parameters were measured and cryosection was analyzed by fluorescence microscopy. Results：The injection was proved successful in all rats in Group ink. Green fluorescence was detected in cryosection of all hearts in Group eGFP and the expression of green fluorescent protein was ubiquitously, but not homongeneous. No rat died during and after the operation among the operated rats（rats in Group eGFP and Group sodium chloride）. There was no difference between hemodynamic parameters of the operated rats and those of the controls. Conclusion：The intrapericardial injection with a trans-diaphragmatic approach is a simple, feasible, safe and valid operation and suitable for small experimental animals. And it suggests an easy, safe, efficient and cheap technique in the study of heart diseases.
出处
《医学研究生学报》
CAS
2005年第10期879-881,i0008,共4页
Journal of Medical Postgraduates
基金
国家自然科学基金资助项目(批准号:30470716)
国家重点基础研究发展规划资助项目(973)(批准号:G2000056906)
关键词
大鼠
注射
心包腔
手术
Rat
Injection
Pericardial sac
Operation
