摘要
目的观察梗阻性黄疸(梗黄)大鼠肠道细菌易位状况及经胃肠道给予精氨酸对肠道细菌易位的影响。方法结扎Wistar大鼠胆管,制成梗黄模型,60只Wistar大鼠随机分为假手术组、梗黄组和梗黄+精氨酸治疗组,每组各20只,于术后21d观察并比较各组血浆内毒素、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的含量及肝功能状况,并对肠系膜淋巴结、胰腺及肺组织行细菌培养,末端小肠做病理检查。结果梗黄组大鼠血浆内毒素、TNF-α、IL-6含量、总胆红素及谷丙转氨酶均明显升高,肠系膜淋巴结、胰及肺组织细菌培养阳性率明显升高,小肠黏膜损伤严重,而精氨酸治疗组以上血清学指标较梗黄组均显著下降,肠系膜淋巴结、胰及肺组织细菌培养阳性率明显降低,小肠黏膜病理损害程度明显减轻。结论梗黄后出现的高内毒素血症与肠道细菌易位关系密切。精氨酸治疗可减轻梗黄时肠道细菌易位,从而降低血浆内毒素水平及细胞因子的过表达。
Objective To observe the conditions of intestinal bacterial transloeation in rat with obstructive jaundice （OJ） and investigate the effects of arginine on bacterial transloeation. Methods The lower eholedoehus was ligated to establish the model of OJ in rats. All the rats were randomized into the sham-operated group, OJ group and arginine group. On postoperative day 21, the changes in levels of plasma endotoxin, TNF-α, IL- 6, total bilirubin and GPT were determined and compared. Bacterial culture of mesenterie lymph nodes, pancreas, and lungs were measured with aseptic manipu lation. The extreme intestinal lumen mueosa was examined pathologically. Results The levels of plas ma endotoxin, TNF-α, IL-6, total bilirubin and GPT were significantly higher in the OJ group than in the sham-operated group. In the OJ group, the positive rate of bacterial culture of mesenterie lymph nodes, pancreas and lungs increased. The pathological damage of intestinal mueosa was severe. The levels of plasma endotoxin, TNF-α, IL- 6, total bilirubin and GPT were markedly lower in the arginine group than in the OJ group. In the arginine group, the positive rate of bacterial culture of the mesenteric lymph nodes, pancreas and lungs dramatically decreased, and the intestinal mueosa injury was remarkably improved. Conclusions Endotoxemia following OJ might be associated with intestinal bacterial transloeation. Arginine is able to alleviate bacterial transloeation from intestinal lumen and reduce the levels of plasma endotoxin and cytokines.
出处
《中华肝胆外科杂志》
CAS
CSCD
2005年第9期630-632,共3页
Chinese Journal of Hepatobiliary Surgery
