摘要
目的：探讨不同种类人乳头状瘤病毒（ＨＰＶ）感染与病变性质的关系。方法：采用聚合酶链反应和Ｓｏｕｔｈｅｒｎ杂交方法，检测尖锐湿疣８９例（尖锐湿疣组）、宫颈癌７６例（宫颈癌组）和正常生殖道１９８例（对照组）组织标本中ＨＰＶ－ＤＮＡ，并对ＨＰＶ感染的主要类型进行分析。结果：尖锐湿疣组中，ＨＰＶ－ＤＮＡ检出率为９８．９％，ＨＰＶ６和ＨＰＶ１１占其中的８６．４％；宫颈癌组中，ＨＰＶ－ＤＮＡ检出率为９４．７％，ＨＰＶ１６和ＨＰＶ１８为主要类型，占８７．５％；对照组中ＨＰＶ隐匿感染率为３７．４％，ＨＰＶ６、１１、１６、１８仅占２１．６％。尖锐湿疣组织中ＨＰＶ１１－ＤＮＡ主要以游离形式存在，而宫颈癌组织中ＨＰＶ１６－ＤＮＡ绝大多数整合到宿主细胞中，并有某种程度的变异。结论：ＨＰＶ的不同类型与病变的组织学损害所表现的特殊形态及性质改变有关。ＨＰＶ－ＤＮＡ与宿主细胞ＤＮＡ的关系及其变异情况，可确定疾病的性质。
Objective:Toexaminehumanpapilomavirus(HPV)infectionrateandtypesinsev-erallesionsofthegenitaltractandtoobservetherelationbetweenHPVandhostcelsfordiferentialhandlingofcasesandforearlydiagnosis.Methods:Tissuesfrom89casesofcondylomaacuminata,76casesofcervicalcancerandswabsfrom198casesofnormalvaginalwereanalysedbypolymerasechainreactionforHPVinfectiverate.MainHPVtypeswerecomparedandSouthernblotwasusedtocomparethephysicalpaternofHPVincondylomaacuminataandcervicalcancer.Results:Theposi-tiveratesofHPV-DNAincondylomaacuminataandcerivcalcancerwererespectively98.9%and94.7%.TheprimarytypesoftheformerwereHPV6,11andofthelattermainly16and18.Thelatentinfectiveratesinnormalvaginawas37.4%,amongwhich,HPV6,11,16,18consistedofonly21.6%.HPV11-DNAincondylomaacuminataexistedmainlyinepisomicform.MostofHPV16-DNAincervicalcancerintegratesintothehostcelswithvariation.Conclusions:ThediferenttypesofHPVarerelatedtoparticularmorphologyandcharacteristicsoflesions.TherelationofHPV-DNAtohostcelandcanofersomereferenceforthedeterminationofthecharacterofthelesion,anditmaybeawardofmalignanttransformation.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
1996年第3期163-165,共3页
Chinese Journal of Obstetrics and Gynecology
关键词
尖锐湿疣
宫颈肿瘤
乳头状瘤病毒
DNA
PCR
CondylomateacuminataCervixneoplasmsPapilomavirusesDNAPolymerasechainreaction
