摘要
目的:探讨宫内缺氧对胚胎大鼠神经干细胞增殖的影响及当归注射液对缺氧神经干细胞的保护作用。方法:实验于2003-09/2004-06在四川省泸州医学院动物科将12只孕鼠随机分为对照组、缺氧组和当归组,每组4只(每只孕鼠产胎鼠5～8只)。当归组孕鼠经尾静脉注入当归注射液,然后用低张性缺氧模型致胎鼠宫内缺氧;缺氧组用生理盐水代替当归注射液,其余同当归组;对照组不缺氧,其余同缺氧组。然后在麻醉状态下处死孕鼠,快速剖腹取胎鼠,每组随机选取20个胚鼠,将各组胎鼠脑组织作神经巢蛋白免疫组化染色,并进行图像分析。结果:60个胎鼠脑室周围脑组织进入结果分析。①缺氧组胎鼠巢蛋白免疫组化反应阳性细胞的吸光度较对照组强(0.262±0.043,0.173±0.021,P<0.05);阳性细胞数量较对照组多[(101.4±15.76),(42.7±6.25)个/视野,P<0.05]。②与缺氧组相比较,当归组胎鼠的巢蛋白免疫组化反应阳性细胞的吸光度减弱(0.222±0.035,P<0.05);阳性细胞数量减少[(58.5±12.53)个/视野,P<0.05]。结论:缺氧后神经干细胞表达巢蛋白增加,当归注射液减弱了缺氧刺激引起的神经干细胞的增殖和分裂,提示当归对缺氧有保护作用。
AIM： To study the effect of intrauterin hypoxia on the proliferation of neural stem cells（NSCs） from embryonic rats and the protective role of Angelica sinensis injection in NSCs in a state of hypoxia. METHODS： The experiment was carried out in the Animal Department of Luzhou Medical College during September 2003 to June 2004. Twelve pregnant Wistar rats were divided randomly into three groups： control group, hypoxia group and Angelica group with four rats in each group（5-8 fetuses for a pregnant rat）. The rats of Angelica group were injected with Angelica sinensis injection through caudal veins before intrauterin hypotonic hypoxia. Normal saline instead of Angelica sincnsis was injected into the rats in the hypoxia group, and other procedures were the same as the Angelica group. No administration of hypoxia was performed in the control group. Under anesthesia, pregnant rats were killed to take rat embryos. Twenty rat embryos were selected randomly in each group to take out brain tissues. The expression of Nestin in brain of embryos was studied with immunohistoehmistry and the results were analyzed by image processing system. RESULTS： Sixty embryos were all involved in the result analysis. ①Absorbance （A） of Nestin positive cells in the hypoxia group was higher than that of the control group （0.262±0.043,0.173±0.021 ,P 〈 0.05）. And the amount of positive cells was also more than that of the control group [（101.4±15.76）,（42.7±6.25）per visual field,P 〈 0.05]. ②Compared with the hypoxia group, the A value of Nestin positive cells in the Angelica group （0.222±0.035,P 〈 0.05）was lower as well as the amount of Nestin positive cells[（58.5±12.53 ）per visual field ,P 〈 0.05]. CONCLUSION： Hypoxia can stimulate the proliferation of the NSCs, and Angelica sinensis injection plays a protective role in the proliferation and differentiation of NSCs from embryonic rats in hypoxia.
出处
《中国临床康复》
CSCD
北大核心
2005年第34期20-22,i0002,共4页
Chinese Journal of Clinical Rehabilitation
基金
四川省教育厅资助课题(2001149)~~
