摘要
目的建立测定口腔溃疡液中氯霉素和盐酸丁卡因含量的HPLC方法.方法以十八烷基硅烷键合硅胶为填充剂;0.8%磷酸∶甲醇∶三乙胺(55∶45∶0.2)为流动相,流速1.0 ml·min-1,检测波长278 nm.结果氯霉素线性关系为Y=11091.8+13173.8X,相关系数r=0.9999(n=5),线性范围在10.16～91.44 g·L-1,平均回收率100.10%(RSD=1.22,n=6);盐酸丁卡因线性关系为Y=4186.65+10132.3X,相关系数r=0.9999(n=5),线性范围在5.98～53.82 g·L-1,平均回收率100.14%(RSD= 1.05% ,n=6).结论该法操作简便、准确,可作为口腔溃疡液中氯霉素和盐酸丁卡因含量的质量控制方法.
Aim An HPLC menthod for the determination of chloramphenical and tetraeation hydroehloride in ulear mouth solutionby was establishad. Methods The amalyti calcolumn C18 themo bide phase of 0.8% phosphoric acid-methanol - triethylamine （55： 45： 0.2） and he flow rate 1.0 ml·min^-1,at the detection wavelength of 78nm. Result The standard curve was linear over the rage of 10.16 -91.44 g·L^-1 of ehloramphenieal with eorreletion enefficient of 0. 9999 （ n = 5 ） ,the average recoveries were ehloramphenical 100. 10 % （ RSD = 1.22% , n = 6 ）. and the standard curve was linear over the rage of 5.98 - 53.82 g·L^-1 of tetraeation hydroehloride with eorreletion enefficient of 0.9999（ n =5 ） ,the average eeoveries werel00.14% （ RSD = 1.05% ,n =6）. Conclusion The method is simple and accurate,it can be used in the quality control of ulear mouth solution.
出处
《安徽医药》
CAS
2005年第11期824-825,共2页
Anhui Medical and Pharmaceutical Journal
