摘要
目的探讨放射标记的膜联蛋白无创检测不稳定斑块的可行性。方法6只新西兰大白兔随机分为实验组和对照组。实验组3只白兔通过球囊拉伤膈下降主动脉内膜,并饲喂含2%胆固醇的高脂饲料15周,制造动脉粥样硬化模型;对照组仅饲喂普通饮食。实验组3只家兔分别注射99TcmannexinⅤ20μg、200μg和500μg,降主动脉活体核素成像和离体核素成像,以及动脉标本数码照相。实验组降主动脉分段,测定其放射强度。结果注射500μg99TcmannexinⅤ5min和120min后,实验性动脉粥样硬化动物沿腹主动脉走行即可见一条放射性浓集的条状影像。离体降主动脉核素成像与大体标本数码照相病变斑块相一致。粥样硬化斑块明显的节段,靶非靶比值也相对较高。结论99TcmannexinⅤ核素成像检测实验性动脉粥样斑块具有一定的可行性,可发展成为一种无创检测不稳定斑块的方法。
Objective To study the feasibility of noninvasive detection of apoptosis in atherosclerotic lesions with radiolabeled annexin V. Methods Atherosclerotic plaques were produced in 3 rabbits by deendothelialization of the infradiaphragmatic aorta followed by 15 weeks of cholesterol diet; 3 rabbits as controls with normal diet without manipulation. Animals were injected with annexin V labeled with technetium-99m before imaging. Aortas were explanted for ex vivo imaging , and photography with digital camera, and annexin uptake analysis. Results There was intense uptake of radiolabel within lesions by 2 hours after injection of ^99Tc^m- annexin V at a dose of 500/μg, no uptake was seen in controls. The results were confirmed in the ex vivo imaging and digital photo of the explanted aorta. Quantitative annexin uptake was higher in lesion versus nonlesion areas. Conclusion Because annexin V could target apoptotic macrophage population, it should constitute an attractive imaging agent for the noninvasive detection of unstable atherosclerotic plaques.
出处
《中国医学影像技术》
CSCD
北大核心
2005年第9期1349-1352,共4页
Chinese Journal of Medical Imaging Technology
