摘要
目的:初步建立人晶状体蛋白质组研究中的双向电泳分离技术,提高晶状体蛋白分辨率和重复性。方法:采用固相pH梯度(IPG)等电聚焦(IEF)为第一向、SDS-PAGE垂直电泳为第二向的双向电泳技术,对样品的处理、水化、等电聚焦、SDS-PAGE等步骤进行了优化。结果:样品经3次重复实验,获得了蛋白质斑点数(133±7)个,蛋白质斑点在IEF方向平均偏差为(2.13±0.17)mm,在SDS-PAGE方向平均偏差为(1.78±0.21)mm,相对百分含量的标准差为(4.01±1.64)%。结论:双向电泳适合于人晶状体蛋白质组的研究。
Objective： To establish and optimize two-dimensional electrophoresis （2-DE） for proteononfic analysis of the human lens and to promote resolution and reproducibility. Methods： A 2-DE technique, including isoelectric focusing （IEF） with an immobilized pH gradient （IPG） and SDS-PAGE, was used. Sample prepantion, rehaydration, SDS-PAGE and other steps were established and optimized. Results： In three, different experiments the total number of protein spots was 133±7, the average deviations in protein position in the. IEF direction was （2.13 ± 0.17）ram and （1.78±0.21） ram in the SDS-PAGE direction. The standard deviation for relative protein volume, was 4.01%±1.64%. Condusion：2-DE meets the. qualifications for for research in burman lens proteome.
出处
《眼视光学杂志》
2005年第3期194-196,共3页
Chinese Journal of Optometry & Ophthalmology
关键词
双向电泳/方法
晶状体/分析
蛋白质/分析
two-dimensional dectrophoresis/methods
lerrq/analysis
proteine/analysis
