摘要
目的探讨金属硫蛋白(MT)和FasL表达与大肠癌淋巴结及肝转移的关系。方法采用免疫组化和定量RT-PCR方法检测93例大肠癌原发灶、正常结肠黏膜、转移淋巴结和肝转移灶MT、FasL表达。结果大肠癌原发灶、正常结肠黏膜、转移淋巴结和肝转移灶中MT表达阳性率分别为:58.1%、32.3%、81.1%、64.3%。FasL表达阳性率分别为:41.9%、19.4%、62.3%、92.9%。癌原发灶、肝转移灶和转移淋巴结中MT、FasL表达阳性率高于正常肠黏膜组织(χ2=35.2421、57.5152,P<0.01)。转移淋巴结MT表达阳性率高于癌原发灶(χ2=8.0565,P<0.01)。肝转移灶FasL表达阳性率高于转移淋巴结和癌原发灶(χ2=8.6674、22.4455,P<0.01)。Dukes′C、D期MT、FasL表达阳性率高于Dukes′A、B期(χ2=18.8871、25.1650,P<0.01)。低分化腺癌和黏液腺癌MT、FasL表达阳性率高于高分化和中分化腺癌(χ2=11.1546、9.2239,P<0.05)。转移淋巴结中MT mRNA表达高于FasL mRNA表达。肝转移灶中FasL mRNA表达水平高于MT mRNA表达。结论MT、FasL增强表达与大肠癌淋巴结转移和肝转移有关,检测MT、FasL表达可作为大肠癌预后评价指标。
Objective To study the expression of metallothionein （MT）and FasL in colorectal cancer and their relation to lymph node and liver metastasis. Methods Immunohistochemistry and quantitative RT-PCR were used to detect expression of MT and FasL in protein and mRNA levels in 93 cases of colorectal cancer. Results The rates of MT expression in primary foci, non-cancerous colon mucosa, lymph node metastasis and liver metastasis were 58. 1% , 32. 3% , 81.1%, 64. 3% respectively. And the rates of FasL expression were 41.9%, 19.4%, 62. 3%, and 92. 9% respectively. The positive rates of MT and FasL in primary foci, liver and lymph node metastasis were higher than that in non-cancerous mucosa （X^2 =35. 2421,57. 5152,P 〈0. 01 ）. MT expression rate in lymph node metastasis was higher than that in primary foci （X^2 =8. 0565 ,P 〈 0.01 ）. In liver metastasis, FasL expression rate was higher than in lymph node metastasis and primary foci （ X^2 =8. 6674,22. 4455 ,P 〈0.01 ）. The positive rates of MT and FasL in Dukes stage C and D were higher than that in Dukes stage A and B （ X^2 = 18. 8871、25. 1650,P 〈0. 01 ）. And higher rates of MT and FasL expression were observed in low differentiation adenocarcinoma and mucus adenocarcinoma than in middle-high differentiation adenocarcinoma （ X^2 = 11. 1546,9. 2239, P 〈 0. 05 ）. High MT mRNA level was found in lymph node metastasis and high FasL mRNA level in liver metastasis. Conclusions Enhanced expression of MT and FasL was associated significantly with lymph node and liver metastasis of colorectal cancer. Assay of MT and FasL expression has prognostic values for colorectal cancer patients.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2005年第17期1118-1120,共3页
Chinese Journal of Surgery
基金
国家自然科学基金资助项目(30271279)
