摘要
mRNA差异显示技术自1992年建立以来,成为分子生物学领域的一个研究热点,并在许多领域得到了广泛应用。本文综述了mRNA差异显示技术的基本原理,其存在的问题及近几年该技术的改进、完善与发展。如引物设计、PCR参数优化、假阳性克服c、DNA片段克隆测序及完整基因的筛选。此外,对其在植物抗寒基因研究中的应用作了简要介绍。
mRNA differential display reverse transcription- PCR（ DDRT- PCR）is a useful technique for analyzing differential gene expression at transcription level. In this paper the theory and characters of mRNA differential display and its application to differentially expressed gene, cold resistance gene cloning, the condition of PCR, primer design and gene sequence inspect are reviewed. The main problem of mRNA differential display and its improved methods are introduced in the paper.
出处
《河北林果研究》
2005年第3期224-227,233,共5页
Hebei Journal of Forestry and Orchard Research
