摘要
目的:观察三叉神经脊束核尾侧亚核(Sp5C)中c-fos(核磷酸蛋白原癌基因)对咬合创伤的反应,探讨神经元在咬合创伤中的功能变化。方法:将直径0.5mm方丝粘固于大鼠左侧上颌第一磨牙,形成左侧磨牙早接触的创伤咬合,观察咬合创伤后1,3,7d,2w,4w,5w时Sp5C中c-fos蛋白的表达变化。结果:正常大鼠Sp5C中偶见极少量c-fos阳性神经元细胞,咬合创伤后7d,c-fos阳性神经元明显增加,2w时达到高峰,4w时开始下降。结论:咬合创伤刺激诱导了Sp5C中神经元的激活。
Objective: To investigate the changes of c-fos in the Sp5C during occlusal trauma and study the effect of occlusal trauma on neurons.Methods: The occlusal surface of the left first maxillary molar was raised 0.5mm with the square wire.On 1,3,7d,2w,4w and 5w after teeth operation,immunofluorescence histochemical was used to examine the number of c-fos contanining neurons in the Sp5C.Results: The number of c-fos-containing neurons increased significantly in experimental side of Sp5C at 7d after occlusal trauma;the maximum number of c-fos contanining neurons was found at 2w after occlusal trauma and returned to the control level at 4w.Conclusion: The neurons in rats’ Sp5C were highly active during occlusal trauma.
出处
《口腔颌面修复学杂志》
2010年第6期338-340,共3页
Chinese Journal of Prosthodontics
基金
国家自然科学基金(项目编号:30772450)
北京市自然科学基金(项目编号:7102165)
