摘要
目的选择合适的大孔树脂,优化工艺参数,分离、纯化金银花叶中的黄酮。方法比较吸附率和解析率,选出合适的大孔树脂HPD100。以产品得率、黄酮得率和黄酮含量为指标,考查最佳工艺条件。结果黄酮粗品拌料与大孔树脂质量比为1∶6,装入柱径与柱长比为1∶6的层析柱中,用2倍柱体积的蒸馏水冲洗,再用70%乙醇以最大流速洗脱,收集2～8倍柱体积的洗脱液,浓缩后得到黄酮产品,得率为4.4%,含量可达91.7%。结论所选工艺操作简单、方法可靠,并能得到高纯度的产品。
OBJECTIVE To choose the suitable macroreticular resin and study the best conditions of separation and purification of the flavonoids in Honeysuckle Leaves ( HL) . METHODS Choosing the suitable macromolecular column to separate,with product yield,flavonids yield,flavonids contents for evaluation system,the optimum conditions were studied. RESULTS Resin HPD100 was the suitable macroreticular resin. The quality of sample and resin was 1∶6,the ratio of diameter and height of loading column was 1∶ 6. Two times o...
出处
《华西药学杂志》
CAS
CSCD
北大核心
2010年第5期575-576,共2页
West China Journal of Pharmaceutical Sciences
基金
宁夏自治区科技攻关项目(编号:06273)
教育部科学技术研究重点项目(编号:03142)
关键词
金银花叶
黄酮
大孔树脂
分离纯化
Honeysuckle Leaves
Flavonoids
Macroreticular resin
Separation and purification
